Donald M Milestone

US Patent:

62074059, Mar 27, 2001

Filed:

Jun 29, 1998

Appl. No.:

9/106615

Inventors:

Shannon L. Bakich - Racine WI
Padma Prabodh Varanasi - Village of Windpoint WI
Donald M. Milestone - Racine WI
Mark M. Gipp - Mount Pleasant WI

Assignee:

S. C. Johnson & Son, Inc. - Racine WI

International Classification:

C12Q1/02
7
C12M1/00

US Classification:

435 29

Abstract:

This invention provides a methodology for rapid biofilm formation in a pilot drain system. In a typical embodiment, aqueous nutrient medium and an inoculum comprising both Sphaerotilus natans and natural biofilm bacteria is utilized to promote growth of a biofilm mass which occupies at least about 20 percent of the conduit volume of a pilot drain system within a time period of about two weeks under simulated drain usage conditions.