Eric M Lam

US Patent:

7015023, Mar 21, 2006

Filed:

May 2, 2000

Appl. No.:

10/009472

Inventors:

Eric Lam - East Brunswick NJ, US
Olga del Pozo - Seville, ES

Assignee:

Rutgers, the State University - New Brunswick NJ

International Classification:

C12P 21/06
C12N 9/00
C12N 9/42
C07H 21/04

US Classification:

435209, 435 4, 435 6, 435 691, 435 697, 435183, 435200, 4352521, 4353201, 435336, 435334, 435219, 536 232, 536 234, 530350

Abstract:

A novel assay system is disclosed for detecting the presence or amount of selected active proteases in biological samples. The assay system utilizes a chimeric protease detector protein composed of three domains: (1) a repressor domain, (2) a protease cleavage domain specific for the protease to be assayed, and a reporter domain. The reporter domain is not detectable when linked to the repressor domain, but becomes detectable upon release from the repressor domain by protease-mediated cleavage. Thus, the activity of the selected protein can be determined by measuring the amount of detectable reporter in the sample. Methods and test kits for using the novel assay system in a variety of in vitro and in vivo applications are also disclosed.

US Patent:

56294700, May 13, 1997

Filed:

Jan 20, 1995

Appl. No.:

8/375778

Inventors:

Eric Lam - East Brunswick NJ
Ron Mittler - Piscataway NJ

Assignee:

Rutgers, The State University of New Jersey - New Brunswick NJ

International Classification:

A01H 500
C12N 504
C12N 1582

US Classification:

800205

Abstract:

Provided by this invention are transgenic higher plants and cells thereof which have been transformed with the bacterio-opsin (bO) gene to provide greater resistance to pathogen attack. Also, provided are bO vectors for forming the transgenic plants and cells thereof. Additionally, a method for enhancing the resistance of higher plants and cells thereof by attack by one or more pathogens is provided.

US Patent:

50231790, Jun 11, 1991

Filed:

Nov 14, 1988

Appl. No.:

7/272169

Inventors:

Eric Lam - New York NY
Philip N. Benfey - New York NY
Philip M. Gilmartin - New York NY
Nam-Hai Chua - Scarsdale NY

International Classification:

C12N 1500
C12N 100
C07H 2104
C12P 2100

US Classification:

4351723

Abstract:

Detection of a cellular factor from pea and tobacco which binds to a repeated pentameric motif of TGACG present in the -90 to -60 region of the CaMV 35S promoter is disclosed. Also disclosed is a 21 bp promoter element which is capable of imparting root expression capability to a rbcS-3A promoter, normally a green tissue specific promoter.

US Patent:

49906070, Feb 5, 1991

Filed:

Mar 14, 1989

Appl. No.:

7/323533

Inventors:

Fumiaki Katagiri - New York NY
Eric Lam - New York NY
Nam-Hai Chua - Scarsdale NY

Assignee:

The Rockefeller University - New York NY

International Classification:

C07H 2104
C21N 500
C21N 1500
C12P 2102

US Classification:

536 27

Abstract:

A transacting DNA binding factor is disclosed. The ASF-1 protein factor specifically binds to the sequence motif TGACG found upstream of the promoter in many plant genes. Co-expression of this protein factor augments the level of expression of the up-regulated promoter containing the TGACG motif.

US Patent:

52234193, Jun 29, 1993

Filed:

Feb 1, 1991

Appl. No.:

7/649521

Inventors:

Fumiaki Katagiri - New York NY
Eric Lam - East Brunswick NJ
Nam-Hai Chua - Scarsdale NY

Assignee:

The Rockefeller University - New York NY

International Classification:

C12N 1567
C12N 1582
C12N 1529

US Classification:

4351723

Abstract:

A transacting DNA binding factor is disclosed. The ASF 1 protein factor specifically binds to the sequence motif TGACG found upstream of the promoter in many plant genes. Coexpression of this protein factor augments the level of expression of the up-regulated promoter containing the TGACG motif.