Gary L Mitchell

US Patent:

42751535, Jun 23, 1981

Filed:

Aug 3, 1978

Appl. No.:

5/930826

Inventors:

Robert J. Gargiulo - Miami FL
Gary A. Mitchell - Miami FL
Patricia M. Hudson - Miami FL
Sharon P. Pochron - Miami FL
Rolf M. Huseby - Miami FL
Robert E. Smith - Livermore CA

Assignee:

American Hospital Supply Corporation - Evanston IL

International Classification:

C12Q 136
C12Q 138
C12Q 156
C07G 700

US Classification:

435 13

Abstract:

Fluorogenic substrates for proteolytic enzymes having the formula: ##STR1## or acid salts thereof wherein: R. sub. 1 is hydrogen-L, hydrogen-D, benzoyl, benzenesulfonyl, glutaryl, pyroglutamyl, carbobenzoxy, D-serine, or carbobenzoxy-serine; R. sub. 2 is hydrogen, phenyl, a straight, branched or cyclic alkyl having 1 to 4 carbons, or propionic acid; R. sub. 3 is hydrogen, straight or branched or cyclic alkyl having 1 to 4 carbons, 4-aminobutane, or 3-guanidylpropane; R. sub. 4 is methyl, 4-aminobutane, or 3-guanidylpropane; R. sub. 5 is a fluorogenic moiety, severable from said compound by a proteolytic enzyme and having different fluorescent property when severed from said compound than when forming part of said compound. The enzymes, when reacting with the substrate, remove the fluorogenic group R. sub. 5 producing an increase in its fluorescence.

US Patent:

46940707, Sep 15, 1987

Filed:

May 28, 1985

Appl. No.:

6/737761

Inventors:

Gary A. Mitchell - Miami Lakes FL
Gerald E. Jaffe - Pembroke Pines FL
Marilyn M. Solorzano - Sunrise FL

Assignee:

Coulter Electronics, Inc. - Hialeah FL

International Classification:

C07K 706
C12Q 138

US Classification:

530329

Abstract:

Water soluble xanthylium derivative substrates of rhodamine 110 and rhodol permit spectrophotometric and fluorescent measurements of trypsin-like enzymes without the addition of organic solvent additives and/or special water solubilizing agents. These novel substrates exhibit increased sensitivity for determining low levels of activity of trypsin-like enzymes such as proteolytic enzymes, cofactors, activators, antiactivators, and inhibitors. These substrates can be substituted for fibrinogen or monitor the pathways of blood coagulation.

US Patent:

43361862, Jun 22, 1982

Filed:

Feb 9, 1981

Appl. No.:

6/232942

Inventors:

Robert J. Gargiulo - Miami FL
Gary A. Mitchell - Miami FL
Patricia M. Hudson - Miami FL
Sharon P. Pochron - Miami FL
Rolf M. Huseby - Miami FL
Robert E. Smith - Livermore CA

International Classification:

C07C10352

US Classification:

2601125R

Abstract:

Fluorogenic substrates for proteolytic enzymes having the formula: ##STR1## or acid salts thereof wherein: R. sub. 1 is hydrogen-L, hydrogen-D, benzoyl, benzenesulfonyl, glutaryl, pyroglutamyl, carbobenzoxy, D-serine, or carbobenzoxy-serine; R. sub. 2 is hydrogen, phenyl, a straight, branched or cyclic alkyl having 1 to 4 carbons, or propionic acid; R. sub. 3 is hydrogen, straight or branched or cyclic alkyl having 1 to 4 carbons, 4-aminobutane, or 3-guanidylpropane; R. sub. 4 is methyl, 4-aminobutane, or 3-guanidylpropane; R. sub. 5 is a fluorogenic moiety, severable from said compound by a proteolytic enzyme and having different fluorescent property when severed from said compound than when forming part of said compound. The enzymes, when reacting with the substrate, remove the fluorogenic group R. sub. 5 producing an increase in its fluorescence.

US Patent:

48015342, Jan 31, 1989

Filed:

Jun 18, 1987

Appl. No.:

7/063353

Inventors:

Gary A. Mitchell - Miami Lakes FL
Gerald E. Jaffe - Pembroke Pines FL
Marilyn M. Solorzano - Sunrise FL

Assignee:

Coulter Electronics, Inc. - Hialeah FL

International Classification:

C12Q 156
C12Q 138
C12N 999

US Classification:

435 13

Abstract:

Water soluble xanthylium derivative substrates of rhodamine 110 and rhodol permit spectrophotometric and fluorescent measurements of trypsin-like enzymes without the addition of organic solvent additives and/or special water solubilizing agents. These novel substrates exhibit increased sensitivity for determining low levels of activity of trypsin-like enzymes such as proteolytic enzymes, cofactors, activators, antiactivators, and inhibitors. These substrates can be substituted for fibrinogen to monitor the pathways of blood coagulation.