Gladis A Walter

Research Fellow

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Location:

Kalamazoo, MI

Industry:

Health, Wellness And Fitness

Work:

University of Michigan - Medical School 2011 - Mar 2013
Research Fellow
University of Michigan Jan 2008 - May 2011
Postdoctoral Researcher at University of Michigan
Oklahoma Medical Research Foundation Mar 2006 - Dec 2007
Postdoctoral Researcher

Education:

Institute of Biomedical Sciences - University of Sao Paulo - USP 2001 - 2005
PhD, Cancer Research, Proteomics University Federal of Parana - UFPR 1997 - 1999
Master's degree, Biochemistry University Federal of Parana
Bachelor's degree, Pharmaceutical Sciences and Biochemistry

Skills:

Biochemistry
Protein Expression
Drug Discovery
Protein Chemistry
Protein Purification
Maldi Tof
Assay Development
Biotechnology
Molecular Biology
Fluorescence Microscopy
Fluorescence Spectroscopy
Fluorescence Anisotropy
Peptides
Protein Folding
Immunology
Lifesciences
Protein Protein Interactions
Small Molecules
Biosensors
High Throughput Screening
Proteomics
Mass Spectrometry
Fplc
Microscopy
Cell
Biophysics
Biomarkers
Pharmacology
Neuroscience
Drug Development
Signal Transduction
Purification
Project Management
Pharmaceutical Sciences
Genomics
Cell Culture
Laboratory
Life Sciences

Interests:

Science and Technology
Social Services
Education

Languages:

English

Awards:

Postdoctoral Research Fellow - T32 - Institutional National Research Service Award (NRSA)
NIH

Gladis Walter Ann Arbor, MI

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Work:

University of Michigan - Medical School
Ann Arbor, MI
Dec 2011 to Mar 2013
Postdoctoral Research Fellow - NIH University of Michigan, Life Sciences Institute, Ann
Ann Arbor, MI
Jan 2008 to Apr 2011
Postdoctoral Research Fellow Oklahoma Medical Research Foundation
Oklahoma City, OK
Mar 2006 to Dec 2007
Postdoctoral Research Associate

Education:

University of So Paulo
So Paulo, SP
Mar 2001 to Jul 2005
Ph.D. in Pharmacology University Federal of Parana - UFPR
Curitiba, PR
1997 to 1999
M.Sc. in Biochemistry University Federal of Parana
Curitiba, PR
1992 to 1997
B.S. in Biochemistry and Pharmaceutical Sciences

Skills:

Molecular Biology: primer design, PCR techniques (RT-PCR, overlap-extension PCR, site-directed mutagenesis); RNA extraction; generation of cDNA constructs encoding fused recombinant proteins and secreted recombinant proteins for expression in Prokaryotic, Yeast or Mammalian expression systems; preparation of chemically competent Escherichia coli and Pichia pastoris cells; E. coli and Pichia pastoris transformation; generation of bicistronic co-expression vectors Protein/enzyme purification, identification and assessment of physical properties, functional conformation and activity: Protein expression (Escherichia coli, Pichia pastoris) and purification applying a variety of chromatographic methods (FPLC-Akta system; HPLC); protease assays using natural substrates or synthetic substrates; protease inhibition assays; active-site titration assays; Fluorescence Spectroscopy (fluorescent probes; anisotropy) and Mass Spectrometry (LC-MS, MALDI-TOF); SDS-PAGE, Western-blot, immunoprecipitation, Co-IP, immunoblotting, biotinylation; Interferometry (BLI-Octet) Experience in enzyme kinetics in order to understand and apply the available technologies Experience with assay development using ELISA and Multiplex Technology, among others Demonstrated success with the development of High Throughput screening assays Good working knowledge of Microsoft suite of Software (Word, Excel), Python, OriginLab, Vector NTI and protein structure analysis programs.