Jason M Tong

US Patent:

20180237841, Aug 23, 2018

Filed:

Feb 15, 2018

Appl. No.:

15/898080

Inventors:

- San Carlos CA, US
Jason TONG - San Carlos CA, US
Xiaoke WANG - San Mateo CA, US

Assignee:

Natera, Inc. - San Carlos CA

International Classification:

C12Q 1/6834

Abstract:

Disclosed here is a composition for isolating nucleic acids from a biological sample, comprising a chaotropic compound and a solvent, wherein the solvent comprises a nitrile compound, tetrahydrofuran, or a combination thereof. Also disclosed is a method for binding nucleic acids to a matrix, comprising: contacting the nucleic acids from a biological sample with the matrix in the presence of a chaotropic compound and a solvent, wherein the solvent comprises a nitrile compound, tetrahydrofuran, or a combination thereof, thereby binding the nucleic acids to the matrix. Further disclosed is a kit for isolating nucleic acids from a biological sample comprising a binding buffer, wherein the binding buffer comprises a chaotropic compound and a solvent, wherein the solvent comprises a nitrile compound, tetrahydrofuran, or a combination thereof.