The present invention relates to recombinant DNA molecules encoding the Tsp45I gene (tsp45IR), and the cognate M. Tsp45I gene (tsp45IM), from Thermus species YS45 introduced into E. coli as well as expression of the recombinant Tsp45I restriction endonuclease in E coli.
Method For Construction Of Thermus-E. Coli Shuttle Vectors And Identification Of Two Thermus Plasmid Replication Origins