Jeffrey C Liu

US Patent:

20200208143, Jul 2, 2020

Filed:

Dec 12, 2019

Appl. No.:

16/712888

Inventors:

- Hayward CA, US
Chenyu LI - Hayward CA, US
Lucie LEE - Union City CA, US
Yang Lily LIU - Oakland CA, US
Jeffrey Juehui LIU - Foster City CA, US

International Classification:

C12N 15/10
C12Q 1/6869

Abstract:

Methods, compositions and systems to amplify short DNA fragments, such as cfDNA, and to reduce random base errors formed in template-dependent primer extension reactions, and to find variant frequencies of mutations on the DNA fragments. The methods, compositions and systems described herein may include, or include the use of, poly(dA) tailing of short DNA fragments by a terminal deoxynucleotidyl transferase, linearly amplification and a multiplex primer extension reaction.

US Patent:

20180195119, Jul 12, 2018

Filed:

Jan 10, 2018

Appl. No.:

15/867031

Inventors:

Zhitong LIU - Foster City CA, US
Guoying LIU - San Ramon CA, US
Jeffrey Juehui LIU - Foster City CA, US
Tao CHEN - San Francisco CA, US

International Classification:

C12Q 1/6874
C12N 15/10
C12Q 1/6806
C12Q 1/6848

Abstract:

Methods, compositions, systems and kits to introduce a controlled-number of molecular barcodes onto DNA fragments by reducing redundant molecular barcodes formed in template-dependent primer extension reactions, and to find variant frequencies from both strands of DNA. The methods, compositions, systems and kits described herein may include, or include the use of, one or more single-stranded DNA specific nucleases that cleave the single-stranded regions containing unmatched base pairs in amplification products.

US Patent:

20170022551, Jan 26, 2017

Filed:

Oct 11, 2016

Appl. No.:

15/290981

Inventors:

Zhitong LIU - Foster City CA, US
Jeffrey Juehui LIU - Foster City CA, US
Guoying LIU - San Ramon CA, US
Tao CHEN - San Francisco CA, US

International Classification:

C12Q 1/68

Abstract:

Methods, compositions, systems and kits to amplify or improve amplification of target-specific amplification products by reducing non-specific amplification products (e.g., primer-dimers) when amplifying multiple different nucleotide regions. The methods, compositions, systems and kits described herein may include, or include the use of, one or more resolvases that recognize and bind to and/or cut an aberrant DNA structure.