A method is presented for the preparation and use of fluorogenic peptide substrates that allows for the configuration of general substrate libraries to rapidly identify the primary and extended specificity of enzymes, such as proteases. The substrates contain a fluorogenic-leaving group, such as 7-amino-4-carbamoylmethyl-coumarin (ACC). Substrates incorporating the ACC leaving group show comparable kinetic profiles as those with the traditionally used 7-amino-4-methyl-coumarin (AMC) leaving group. The bifunctional nature of ACC allows for the efficient production of single substrates and substrate libraries using solid-phase synthesis techniques. The approximately 3-fold increased quantum yield of ACC over AMC permits reduction in enzyme and substrate concentrations. As a consequence, a greater number of substrates can be tolerated in a single assay, thus enabling an increase in the diversity space of the library. Soluble positional protease substrate libraries of 137,180 and 6,859 members, possessing amino acid diversity at the P4-P3-P2-P1 and P4-P3-P2 positions, respectively, were constructed.
Tryptase Substrates And Assay For Tryptase Activity Using Same
Andrew L. Niles - Madison WI Mary Haak-Frendscho - Newark CA Jennifer L. Harris - San Diego CA Charles S. Craik - San Francisco CA
Assignee:
Promega Corporation - Madison WI
International Classification:
C12Q 100
US Classification:
435 4, 435 23, 530324, 530330
Abstract:
The invention is directed to synthetic polypeptide substrates for tryptase enzymes and assays for tryptase activity that utilize the synthetic polypeptide substrates. The preferred synthetic polypeptide substrates are tetramers of the formula P4-P3-P2-P1, wherein the substrate is selected from the group consisting of P-R-N-K (SEQ. ID. NO: 2), P-K-N-K (SEQ. ID. NO: 3), P-R-N-R (SEQ. ID. NO: 4), P-K-N-R (SEQ. ID. NO: 5), P-A-N-K (SEQ. ID. NO: 6), and P-R-T-K (SEQ. ID. NO: 7).
Hong Liu - San Diego CA, US David Tully - San Diego CA, US Robert Epple - San Diego CA, US Badry Bursulaya - San Diego CA, US Jennifer Williams - La Jolla CA, US Arnab Chatterjee - Encinitas CA, US Jennifer Leslie Harris - San Diego CA, US Jun Li - San Diego CA, US
The present invention provides compounds, compositions and methods for the selective inhibition of cathepsin S. In a preferred aspect, cathepsin S is selectively inhibited in the presence of at least one other cathepsin isozyme (e. g. , cathespin K). The present invention also provides methods for treating a disease state in a subject by selectively inhibiting cathepsin S.
Hong Liu - San Diego CA, US Phil Alper - Poway CA, US Arnab Chatterjee - Encinitas CA, US David Tully - San Diego CA, US Badry Bursulaya - San Diego CA, US David Woodmansee - San Diego CA, US Robert Epple - San Diego CA, US Jennifer Leslie Harris - San Diego CA, US Jun Li - San Diego CA, US
Assignee:
IRM LLC - Hamilton
International Classification:
A61K 31/4045 C07D 209/08
US Classification:
514415, 548491
Abstract:
The present invention provides compounds, compositions and methods for the selective inhibition of cathepsin S. In a preferred aspect, cathepsin S is selectively inhibited in the presence of at least one other cathepsin isozyme. The present invention also provides methods for treating a disease state in a subject by selectively inhibiting cathepsin S.
A method is presented for the preparation and use of fluorogenic peptide substrates that allows for the configuration of general substrate libraries to rapidly identify the primary and extended specificity of enzymes, such as proteases. The substrates contain a fluorogenic-leaving group, such as 7-amino-4-carbamoylmethyl-coumarin (ACC). Substrates incorporating the ACC leaving group show comparable kinetic profiles as those with the traditionally used 7-amino-4-methyl-coumarin (AMC) leaving group. The bifunctional nature of ACC allows for the efficient production of single substrates and substrate libraries using solid-phase synthesis techniques.
Bradley Backes - Chicago IL, US Jennifer Harris - San Diego CA, US
Assignee:
IRM, LLC - Hamilton
International Classification:
G01N033/53 C12Q001/37 C07K007/06 C07K007/08
US Classification:
435/007100, 435/023000, 530/324000
Abstract:
Non-peptide protease substrate libraries and high purity protease substrate libraries are constructed, e.g., using fluorogenic compounds. The libraries are useful in obtaining substrate profiles for a variety of proteases, such as methods for determining both prime and non-prime protease recognition sequences.
Substrate specificity profiles are used to determine optimal hepsin substrate sequences, both to the prime side and non-prime side of the hepsin recognition site. The hepsin substrate sequences are used in designing substrates, inhibitors, and prodrugs. For example, prodrugs are provided for use in the treatment of prostate cancer. Hepsin inhibitors based on substrate specificity are also provided.
Jennifer Harris - San Diego CA, US Aaron Shipway - Cardiff CA, US
Assignee:
IRM LLC - Hamilton
International Classification:
C12Q001/37 C12Q001/18
US Classification:
435023000, 435032000
Abstract:
This invention provides novel methods for identifying modulators of transmembrane protease serine 6 (TMPRSS6). The methods comprise screening test agents for ability to modulate proteolysis of a pathogenic toxin substrate or a synthetic peptide substrate of TMPRSS6. The methods can further comprise screening the identified modulating agents for ability to inhibit infections of pathogens. Also provided in the invention are methods and pharmaceutical compositions for treating infections of pathogens whose toxins are proteolytically activated by TMPRSS6.
Our Lady Of The Lake Physician Group 12525 Perkins Rd STE C, Baton Rouge, LA 70810 2257692003 (phone), 2257673055 (fax)
Education:
Medical School Johns Hopkins University School of Medicine Graduated: 1998
Procedures:
Hearing Evaluation Vaccine Administration
Conditions:
Abdominal Hernia Anemia Chronic Sinusitis Croup Otitis Media
Languages:
English French Russian Spanish
Description:
Dr. Harris graduated from the Johns Hopkins University School of Medicine in 1998. She works in Baton Rouge, LA and specializes in Pediatrics and Adolescent Medicine. Dr. Harris is affiliated with Our Lady Of The Lake Regional Medical Center and Womens & Childrens Hospital.
Samuel Y Brown Pediatrics 3813 Williams Blvd, Kenner, LA 70065 5044435437 (phone), 5044432272 (fax)
Languages:
English Spanish
Description:
Ms. Harris works in Kenner, LA and specializes in Pediatrics. Ms. Harris is affiliated with East Jefferson General Hospital and Ochsner Medical Center Jefferson Highway.
General Practice Landlord & Tenant Personal Injury Social Security Administrative & Regulatory Law Alternative Dispute Resolution Business Law Information Technology Law Criminal Law Family Law Labor & Employment Law Law Practice Management & Legal Administrators Negligence Law Animal Law Consumer Law International Law Childrens Law Marijuana Law LGBTQA Law Probate & Estate Planning Real Property Law Elder Law & Disability Rights
Mar 2013 to 2000 Director of Internal & External GrantsJACKLEG PRESS
2011 to 2000 Founder and editor-in-chief of a small publishing pressHINMAN FOUNDATION
Oct 2012 to Dec 2012 ConsultantFoundation and Government Grants, Alumni Relations, Annual Fund
May 2008 to Jul 2011 Executive Director of Institutional AdvancementNATIONAL LOUIS UNIVERSITY
Jul 2007 to May 2008 Executive DirectorNATIONAL LOUIS UNIVERSITY
2007 to 2008 ChairBoard of Trustees
Aug 2003 to Jun 2007 Director of Corporate and Foundation RelationsNATIONAL LOUIS UNIVERSITY
2007 to 2007 Adjunct ProfessorTHE CHICAGO COUNCIL ON GLOBAL AFFAIRS
2002 to 2003 Director of Special Events and Travel ProgramPEGGY NOTEBAERT NATURE MUSEUM
2001 to 2002 Senior Grants OfficerILLINOIS INSTITUTE OF TECHNOLOGY
Jan 2000 to Jun 2001 Director of DevelopmentILLINOIS INSTITUTE OF TECHNOLOGY
May 2000 to Jan 2001 Associate DirectorILLINOIS INSTITUTE OF TECHNOLOGY
2000 to 2001THE ART INSTITUTE OF CHICAGO
1995 to 2000 Grants Coordinator, Government RelationsDEMOCRATIC NATIONAL COMMITTEE Washington, DC 1991 to 1993 Deputy Director of Training Division and the Job Bank
Education:
National Louis University Aug 2012 PhD in PsychologyThe School of the Art Institute of Chicago Dec 1999 MFAUniversity of Arizona May 1993 BFA in Creative Writing
CDC Atlanta, GA Dec 2012 to Jun 2014 Human Resources SpecialistUSAF
Mar 2009 to Dec 2012 Human Resources SpecialistCompensation, CPMS Arlington, VA Jul 2005 to Mar 2009 Human Resources SpecialistHuman Resources (Military)
Jun 2004 to Jul 2005 ComptrollerMilitary Personnel
Sep 1977 to Dec 1993
Education:
Barry University May 2004 Bachelor's in Human Resources
2010 to 2000 Executive AssistantKeane Federal Systems/Federal Bureau of Investigation Washington, DC 2008 to 2010 Administrative AnalystPitney Bowes Government Solutions Capitol Heights, MD 2005 to 2008 Clerk IIGreenfield-Caputo M.D Annapolis, MD 2004 to 2005 Clinical/Clerical Medical AssistantHome Furnishings for Less Bowie, MD 2001 to 2004 Secretary
Education:
Prince Georges Community College Largo, MD 2003 to 2004 Business AdministrationGwynn Park High School Upper Marlboro, MD 1999 to 2003 High School DiplomaAnne Arundel Community College Arnold, MD Associate of Science in Nursing
Skills:
Microsoft Office Suite: MS Word, MS Excel, PowerPoint, Outlook; Windows XP/Windows 7 Typing Speed: 85 WPM
Apex Systems Fort Belvoir, VA May 2013 to Oct 2013 Document ScannerAmerican Systems Lorton, VA May 2011 to Jan 2012 Call Center RepresentativeChronos Systems Arlington, VA Apr 2010 to May 2011 Document Scanner
Education:
Northern Virginia Community College Woodbridge, VA 2013 to 2016 Associates in Information TechnologyGARFIELD HS-Woodbridge Jacksonville, SC 2004 to 2008 Certificate in BASIC TRAINING
Aug 2014 to 2000 Quality Improvement CoordinatorHoward University Hospital (Allied Health Staffing)
Sep 2013 to Aug 2014 Audit Management Analyst (Revenue Cycle Integrity)Delmarva Foundation for Medical Care Columbia, MD Jun 2012 to Sep 2012 Quality Improvement Consultant IIDelmarva Foundation for Medical Care Columbia, MD Aug 2011 to Sep 2012 UR Nurse/Review Associate IIWashington Adventist Hospital Takoma Park, MD Mar 2007 to Feb 2012 Clinical RN-Special Care
Adecco ( Blue Linx Corporation) Atlanta, GA Mar 2013 to Aug 2013 Accounting AssistantRandstand (State Farm) Alpharetta, GA Oct 2012 to Mar 2013 Data Entry ClerkAT&T Atlanta, GA Oct 2009 to Oct 2012 Customer Support RepresentativeWal-Mart Marietta, GA 2007 to 2009 Customer Service /Sales Associate
Education:
University of Phoenix Atlanta, GA Financial Planning & Business MarketingClark Atlanta University Atlanta, GA May 2008 Business AdministrationBenjamin E Mays High School Atlanta, GA May 2007 diploma
Thomas Jefferson School Alton IL 1976-1978, Horace Mann Elementary School Alton IL 1979-1983, East Junior High School Alton IL 1983-1986, East Middle School Alton IL 1983-1986
Biography:
Life
Married with 3 boys. My oldest is now 17. I am still married to Greg. We are ...
Youngstown Elementary School Edmonton Azores 1980-1987, Afton Elementary School Edmonton Azores 1980-1987, Britannia Junior High School Edmonton Azores 1987-1989, Wellington Junior High School Edmonton Azores 1987-1990, Ecole Secondaire Beaumont High School Beaumont Azores 1987-1993
Community:
Stella Nguyen, Brent Chalmers, Shirley Johnson
Biography:
Life
I have been married for 7 years, 2 Kids (1 girl, 1 Boy). Currently living in E...
Phoenix, AZ Norfolk, VA Pensacola, FL Lakeside, CA El Cajon, CA
Work:
Arizona House of Representatives - Minority Policy Advisor (2012) Arizona State Senate - Assistant (2006-2012)
Education:
Northern Arizona University - Health Sciences, Grossmont College - General Studies
Jennifer Harris
Work:
The Travel Connection - Disney Travel Agent YMCA of Roanoke Valley - Youth and Family Stonegate Swim Club - Hatchlings Swim Coach Kirk Family YMCA - Aquatics (2013) Glenvar High School - Swim Coach (2013)
Education:
Mary Baldwin College
About:
I am an Independent Travel Agent who specializes in planning Disney vacations! I am with The Travel Connection. I prefer to think of myself as a vacation planner. Or magic maker. Or maker of your Disn...
Jennifer Harris
Lived:
Encinitas, CA Atlanta, GA Washington, DC
Work:
Highheelgolfer, LLC - Founder (2012)
Education:
Ohio State University - Psychology, Marketing
Bragging Rights:
Lowest 18 hole score = 65
Jennifer Harris
Work:
Atlantic Apothecary - Pharmacy Technician (2010)
Education:
Washington College - Pre-pharmacy, Smyrna High School
Tagline:
Hi :D I'm Jenn. I have an obsession with my cat, Taylor Swift, and the color yellow. Get at me.
Bragging Rights:
Im a ginger, what else is there to brag about?
Jennifer Harris
Work:
Festivolve - Green Events Coordinator Freestone Fermentation Festival - Event Coordinator
Education:
Hutchins Institute - Liberal Studies
Relationship:
In_a_relationship
About:
Thought is the blossom, language the bud, action the fruit behind it. -Ralph Waldo Emerson
Jennifer Harris
Education:
Capital Community College - Business, Bulkeley high school
Relationship:
In_a_relationship
About:
I love all my children and enjoy staying home taking care of my babies, and my man.
Bragging Rights:
I have 3 beautiful kids and I am courantly taking colleges courses in business
Jennifer Harris
Lived:
Atlanta, GA
Work:
Georgia State University--Department of Biology - Accountant III
I am an Executive Search consultant for Stelfox UK who specialise in Global Pharmaceutical Executive Search. I head up the area of Medical and Healthcare... I am an Executive Search consultant for Stelfox UK who specialise in Global Pharmaceutical Executive Search. I head up the area of Medical and Healthcare Communications working on positions in UK, Europe and the US. I am currently recruiting for positions from Medical Writer to Client Services...
It's a weird day when I agree with Steve Bannon, but he's right on this, says Jennifer Harris, a China expert at the Council on Foreign Relations and a former top staffer in President Obama's State Department. Going back to George W. Bush, America's policy toward China has been to ask nicely. Tha
Date: Aug 18, 2017
Category: Top Stories
Source: Google
Ex-Clinton Aide Warns Next President To Watch Out For 'Chinese Economic Coercion'
American geopolitical strategists need to take a bit more seriously than it is currently just how much of the Chinese coercion is taking an economic form, Jennifer Harris, a Council on Foreign Relations (CFR) expert and former member of the U.S. Department of States policy planning staff, told th
Date: Sep 29, 2016
Category: World
Source: Google
China invests in UK as 'special relationship' with USA falters
"The British are most inclined to be optimistic on where China is headed, given their economic and financial incentives are stronger than other EU countries," said Jennifer Harris, a senior fellow at the Council on Foreign Relations formerly with the State Department and the National Intelligence Co
Date: Jun 29, 2016
Category: World
Source: Google
Astronaut twins study raises questions about genetic privacy
By seeing which genes are turned on or turned off in orbit, researchers could better understand the genetic pathways that underlie physiological changes, says Jennifer Harris, a genetic epidemiologist who specializes in twin studies1 at the Norwegian Institute of Public Health in Oslo who is not inv
Director of marketing initiatives at Rudd Center and author of the study Jennifer Harris says, "Although many parents know that soda is not good for children, many still believe that sugary drinks are healthy options. The labeling and marketing for these products imply that they are nutritious, and
"Although many parents know that soda is not good for children, many still believe that sugary drinks are healthy options," says Jennifer Harris, who wrote the study and is director of marketing initiatives at Rudd Center. "The labeling and marketing for these products imply that they are nutritious
Date: Mar 11, 2015
Category: Health
Source: Google
Sugary Drinks a Healthy Option? Most Parents Think So
incarnations of sugary drinks, according to a new study released Wednesday by the Rudd Center for Food Policy & Obesity at the University of Connecticut. Many still believe that other sugary drinksfruit drinks, sports drinks and flavored waterare healthy options, said Jennifer Harris, Ph.D, a stu
"A lot of marketing is designed to give the impression that these are healthy drinks," said Jennifer Harris, director of marketing initiatives at UConn's Rudd Center for Food Policy and Obesity, which conducted the study.
Date: Mar 11, 2015
Category: Health
Source: Google
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Subscribe to #NuLife Christian Fellowship Church channel to stream our...