President and Fellows of Harvard College - Cambridge MA, US Jing Liu - Brookline MA, US
Assignee:
President and Fellows of Harvard College - Cambridge MA
International Classification:
C12Q 1/02
US Classification:
435 29, 536 2722, 530409, 435325
Abstract:
Synthesis of many proteins is tightly controlled at the level of translation and plays an essential role in fundamental processes such as cell growth and proliferation, signaling, differentiation or death. Methods that allow imaging and identification of nascent proteins allow for dissecting regulation of translation, both spatially and temporally, including in whole organisms. Described herein are robust chemical methods for imaging and affinity-purifying nascent polypeptides in cells and in animals, based on puromycin analogs. Puromycin analogs of the present invention form covalent conjugates with nascent polypeptide chains, which are rapidly turned over by the proteasome and can be visualized and specifically captured by a bioorthogonal reaction (e.g., [3+2]cycloaddition). The methods of the present invention have broad applicability for imaging protein synthesis and for identifying proteins synthesized under various physiological and pathological conditions in vivo.
- Boston MA, US - New York NY, US Husnu Umit KANISKAN - New York NY, US He CHEN - New York NY, US Jing LIU - Boston MA, US
Assignee:
Beth Israel Deaconess Medical Center, Inc. - Boston MA Icahn School of Medicine at Mount Sinai - New York NY
International Classification:
A61K 41/00 A61K 47/55 A61K 47/54 A61P 35/00
Abstract:
By hijacking endogenous E3 ligase to degrade protein targets via the ubiquitin-proteasome system, PROTACs (PRoteolysis TArgeting Chimeras) provide a new strategy to inhibit protein targets that were previously regarded as undruggable. The compounds described herein comprise a photolabile group on PROTACs, enabling the degradation of protein targets in a spatiotemporally controlled manner By adding a photolabile caging group on ubiquitin recruiting moieties, light-inducible protein degradation was acheived. These opto-PROTACs display no activity in the dark, while restricted degradation can be induced at a specific time and rate by UVA-irradiation. Accordingly, these compounds provide light-controlled PROTACs and methods of using such compounds.
Methods And Compositions For Labeling Polypeptides
President and Fellows of Harvard College - Cambridge MA
International Classification:
C07K 14/00 G01N 33/58
Abstract:
Synthesis of many proteins is tightly controlled at the level of translation and plays an essential role in fundamental processes such as cell growth and proliferation, signaling, differentiation or death. Methods that allow imaging and identification of nascent proteins allow for dissecting regulation of translation, both spatially and temporally, including in whole organisms. Described herein are robust chemical methods for imaging and affinity-purifying nascent polypeptides in cells and in animals, based on puromycin analogs. Puromycin analogs of the present invention form covalent conjugates with nascent polypeptide chains, which are rapidly turned over by the proteasome and can be visualized and specifically captured by a bioorthogonal reaction (e.g., [3+2] cycloaddition). The methods of the present invention have broad applicability for imaging protein synthesis and for identifying proteins synthesized under various physiological and pathological conditions in vivo.
Sep 2010 to 2000 Postdoctoral Research Fellow, Lab of Genetic NeuropharmacologyCeleros seperations Inc Foxborough, MA 2009 to 2010 Scientist/ ConsultantBrown University, Division of Biology Providence, RI 2004 to 2006 Teaching AssistantXian - Janssen Pharmaceutical Ltd GuangZhou, CN 2001 to 2002 Medical Associate, Sales DepartmentMeiRui Pharma Co., Ltd Nanjing, CN 1998 to 2000 Project Associate/Analyst, R&B Department
Education:
Brown University Providence, RI 2009 Ph.D. in Molecular PharmacologyChina Pharmaceutical University Nanjing, CN 1998 B.S. in Human Pharmacology
To learn more about potentially habit-forming sleep aids, we spoke to Dr. Jing Liu, OMD, PHD of Sol Nutrition. She told us that among others, melatonin and sleep aids containing antihistamines may lead to dependency. Learn more about these supplements and their potential side effects below.
Date: Apr 19, 2023
Category: Health
Source: Google
Chinese COVID-19 Vaccine Phase 2 Trial Results: Safe and Induces an Immune Response
Just like what the Terminator does in the Hollywood sci-fi film, this soft machine appears rather intelligent and can transform itself depending on the space it occupies, according to Jing Liu from the Tsinghua University This unusual behavior in robots perfectly resembles how living organisms react
Date: Mar 16, 2015
Category: Sci/Tech
Source: Google
Future T-1000 robots applaud as scientists invent self-powered liquid metal
"The soft machine looks rather intelligent and can deform itself according to the space it voyages in, just like Terminator does from the science-fiction film," explained Tsinghua University scientist Jing Liu. "These unusual behaviors perfectly resemble the living organisms in nature."
Date: Mar 12, 2015
Category: Sci/Tech
Source: Google
3-D Laser Map Shows Earthquake Zone Before and After
Other co-authors of the paper are: Austin Elliott and Peter Gold, UC Davis; J. Ramon Arrowsmith, Arizona State University; Alejandro Hinojosa Corona and J. Javier Gonzalez Garcia, CICESE, Mexico; Eric Fielding, NASA Jet Propulsion Laboratory; and Jing Liu-Zeng, Chinese Academy of Sciences.
Date: Feb 10, 2012
Category: Sci/Tech
Source: Google
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Jing Liu (Amanda)
Work:
Oracle Corporation - Software Engineer (2013)
Education:
University of Pennsylvania - TCOM, Southeast University - Computer Science & Engineering, Chengdu Foreign Languages School - Senior High School, Chengdu Foreign Languages School - Junior High School
Jing Liu
Education:
Panjin Vocational and Technical College - Communication network and equipment