John W Gruber

US Patent:

20130177962, Jul 11, 2013

Filed:

Mar 12, 2013

Appl. No.:

13/796985

Inventors:

John M. Gruber - El Dorado Hills CA, US
Emily Mundorff - Garden City NY, US
Gjalt W. Huisman - San Carlos CA, US
Steven James Collier - Singapore, SG

Assignee:

CODEXIS, INC. - Redwood City CA

International Classification:

C12N 9/00

US Classification:

435190, 536 232, 4353201, 43525233

Abstract:

The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N,N-dimethyl-3-keto-3-(2-thienyl)-1-ketopropanamine to (S)—N,N-dimethyl-3-hydroxy-3-(2-thienyl)-1-propanamine.

US Patent:

20130196408, Aug 1, 2013

Filed:

Mar 11, 2013

Appl. No.:

13/793158

Inventors:

John M. Gruber - El Dorado Hills CA, US
Gjalt W. Huisman - San Carlos CA, US
Emily Mundorff - Garden City NY, US
Lisa M. Newman - San Jose CA, US

Assignee:

CODEXIS, INC. - Redwood City CA

International Classification:

C12N 9/00

US Classification:

435190, 536 232, 4353201, 43525233

Abstract:

The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.

US Patent:

20230104779, Apr 6, 2023

Filed:

Oct 26, 2022

Appl. No.:

18/049758

Inventors:

- REDWOOD CITY CA, US
John M. Gruber - El Dorado Hills CA, US
Emily Mundorff - Garden City NY, US
Gjalt W. Huisman - Redwood City CA, US
Steven J. Collier - Concord MA, US

International Classification:

C12P 17/00
C12N 9/04
C12P 13/00

Abstract:

The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N-methyl-3-keto-3-(2-thienyl)-1-propanamine to (S)—N-methyl-3-hydroxy-3-(2-thienyl)-1-propanamine.

US Patent:

20200340025, Oct 29, 2020

Filed:

Jul 17, 2020

Appl. No.:

16/932084

Inventors:

- Redwood City CA, US
John M. Gruber - El Dorado Hills CA, US
Emily Mundorff - Garden City NY, US
Gjalt W. Huisman - Redwood City CA, US
Steven J. Collier - Concord MA, US

International Classification:

C12P 17/00
C12N 9/04
C12P 13/00

Abstract:

The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N-methyl-3-keto-3-(2-thienyl)-1-propanamine to (S)—N-methyl-3-hydroxy-3-(2-thienyl)-1-propanamine.

US Patent:

20180251802, Sep 6, 2018

Filed:

May 17, 2018

Appl. No.:

15/982291

Inventors:

- Redwood City CA, US
John M. Gruber - El Dorado Hills CA, US
Emily Mundorff - Garden City NY, US
Gjalt W. Huisman - Redwood City CA, US
Steven J. Collier - Concord MA, US

International Classification:

C12P 17/00
C12P 13/00
C12N 9/04

Abstract:

The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N-methyl-3-keto-3-(2-thienyl)-1-propanamine to (S)—N-methyl-3-hydroxy-3-(2-thienyl)-1-propanamine.

US Patent:

20170226546, Aug 10, 2017

Filed:

Apr 18, 2017

Appl. No.:

15/489946

Inventors:

- Redwood City CA, US
John M. Gruber - El Dorado Hills CA, US
Emily Mundorff - Garden City NY, US
Gjalt W. Huisman - Redwood City CA, US
Steven J. Collier - Concord MA, US

International Classification:

C12P 17/00
C12P 13/00
C12N 9/04

Abstract:

The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N-methyl-3-keto-3-(2-thienyl)-1-propanamine to (S)—N-methyl-3-hydroxy-3-(2-thienyl)-1-propanamine.

US Patent:

20160083759, Mar 24, 2016

Filed:

Nov 30, 2015

Appl. No.:

14/954564

Inventors:

- REDWOOD CITY CA, US
John M. Gruber - El Dorado Hills CA, US
Emily Mundorff - Garden City NY, US
Gjalt W. Huisman - Redwood City CA, US
Steven J. Collier - Concord MA, US

International Classification:

C12P 17/00
C12N 9/04

Abstract:

The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N-methyl-3-keto-3-(2-thienyl)-1-propanamine to (S)—N-methyl-3-hydroxy-3-(2-thienyl)-1-propanamine.

US Patent:

20150031094, Jan 29, 2015

Filed:

Oct 1, 2014

Appl. No.:

14/503578

Inventors:

- Redwood City CA, US
John M. Gruber - El Dorado Hills CA, US
Emily Mundorff - Garden City NY, US
Gjalt W. Huisman - Redwood City CA, US
Steven J. Collier - Concord MA, US

International Classification:

C12P 13/00
C12N 9/04

US Classification:

435128, 435190, 43525233, 4353201, 536 232

Abstract:

The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N-methyl-3-keto-3-(2-thienyl)-1-propanamine to (S)—N-methyl-3-hydroxy-3-(2-thienyl)-1-propanamine.