John Thomas Gruber

US Patent:

6469181, Oct 22, 2002

Filed:

Jan 30, 1995

Appl. No.:

08/380765

Inventors:

John M. Gruber - Mountain View CA
A. Ray Bulls - Sunnyvale CA
John H. Grate - Mountain View CA

Assignee:

Catalytica, Inc. - Mountain View CA

International Classification:

C07D20934

US Classification:

548486, 560 23

Abstract:

The present invention provides a processes, having practical utility, for preparing 2-oxindoles, N-hydroxy-2-oxindoles, or mixtures thereof comprising: catalytically hydrogenating a 2-nitroarylmalonate diester to produce a 2-(N-hydroxyamino)arylmalonate diester, a 2-aminoarylmalonate diester, or mixtures thereof as a first reaction intermediate; cyclizing, by intramolecular aminolysis of one ester group, the first reaction intermediate to produce a N-hydroxy-2-oxindole-3-carboxylate ester, 2-oxindole-3-carboxylate ester, or mixtures thereof as a second reaction intermediate; and hydrolyzing and decarboxylating the remaining ester group of the second reaction intermediate to produce the N-hydroxy-2-oxindole, the 2-oxindole, or mixtures thereof, wherein the cyclization reaction and the hydrolysis and decarboxylation reaction are conducted in situ with the catalytic hydrogenation reaction without isolation of said reaction intermediates.

US Patent:

7820421, Oct 26, 2010

Filed:

Feb 8, 2008

Appl. No.:

12/028780

Inventors:

Charlene Ching - San Jose CA, US
John M. Gruber - El Dorado Hills CA, US
Gjalt W. Huisman - San Carlos CA, US
Emily Mundorff - Belmont CA, US
Lisa M. Newman - San Jose CA, US

Assignee:

Codexis, Inc. - Redwood City CA

International Classification:

C12N 9/04
C12N 1/20
C12N 15/00
C12Q 1/00
C12Q 1/68
C12Q 1/34
C12P 21/04
C07H 21/04

US Classification:

435190, 435 4, 435 6, 435 18, 435440, 435 691, 435 711, 4352523, 4353201, 536 232

Abstract:

The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.

US Patent:

8071347, Dec 6, 2011

Filed:

Sep 14, 2010

Appl. No.:

12/881734

Inventors:

Charlene Ching - San Jose CA, US
John M. Gruber - Mountain View CA, US
Gjalt W. Huisman - San Carlos CA, US
Emily Mundorff - Belmont CA, US
Lisa M. Newman - San Jose CA, US

Assignee:

Codexis, Inc. - Redwood City CA

International Classification:

C12N 9/04
C12N 1/20
C12N 15/00
C12Q 1/00
C12Q 1/34
C12P 21/04
C07H 21/04

US Classification:

435190, 435 4, 435 18, 435440, 4352523, 4353201, 435 691, 435 711, 536 232

Abstract:

The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.

US Patent:

8178333, May 15, 2012

Filed:

Jun 23, 2009

Appl. No.:

12/490190

Inventors:

Benjamin Mijts - Belmont CA, US
Sheela Muley - Fremont CA, US
Jack Liang - San Mateo CA, US
Lisa M. Newman - San Jose CA, US
Xiyun Zhang - Fremont CA, US
James Lalonde - Palo Alto CA, US
Michael D. Clay - Menlo Park CA, US
Jun Zhu - Sunnyvale CA, US
John M. Gruber - El Dorado Hills CA, US
Jeffrey Colbeck - Menlo Park CA, US
Jagadeesh Mavinahalli - Singapore, SG
Roger Sheldon - Hoog Keppel, NL

Assignee:

Codexis, Inc. - Redwood City CA

International Classification:

C12N 9/00
C07H 21/04

US Classification:

435183, 435 691, 4353201, 536 231

Abstract:

The present disclosure provides monoamine oxidase enzymes and methods of their use in biocatalytic processes for the preparation of substantially enantiomerically pure heterobicyclic compounds of the following structural formulas,.

US Patent:

8288141, Oct 16, 2012

Filed:

Aug 27, 2009

Appl. No.:

12/549293

Inventors:

Christopher Savile - Sunnyvale CA, US
John M. Gruber - El Dorado Hills CA, US
Emily Mundorff - Belmont CA, US
Gjalt Huisman - San Carlos CA, US
Steven James Collier - Singapore, SG

Assignee:

Codexis, Inc. - Redwood City CA

International Classification:

C12N 9/02
C12N 9/04
C12P 17/12
C12P 13/00
C07H 21/04

US Classification:

435189, 435190, 435122, 435128, 536 232

Abstract:

The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N-methyl-3-keto-3-(2-thienyl)-1-propanamine to (S)—N-methyl-3-hydroxy-3-(2-thienyl)-1-propanamine.

US Patent:

8415127, Apr 9, 2013

Filed:

Nov 7, 2011

Appl. No.:

13/290773

Inventors:

Charlene Ching - San Jose CA, US
John M. Gruber - El Dorado Hills CA, US
Gjalt W. Huisman - San Carlos CA, US
Emily Mundorff - Poughkeepsie NY, US
Lisa M. Newman - San Jose CA, US

Assignee:

Codexis, Inc. - Redwood City CA

International Classification:

C12N 9/04
C12N 1/20
C12N 15/00
C12Q 1/00
C12Q 1/34
C12P 21/04

US Classification:

435190, 435 4, 435 18, 435440, 4352523, 4353201, 435 691, 435 711

Abstract:

The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.

US Patent:

8426178, Apr 23, 2013

Filed:

Aug 27, 2009

Appl. No.:

12/549154

Inventors:

Christopher Savile - Sunnyvale CA, US
John M. Gruber - El Dorado Hills CA, US
Emily Mundorff - Belmont CA, US
Gjalt Huisman - San Carlos CA, US
Steven James Collier - Singapore, SG

Assignee:

Codexis, Inc. - Redwood City CA

International Classification:

C12N 9/02
C12P 13/00
C12Q 1/26
C07K 14/00
C12P 21/00

US Classification:

435189, 435128, 435 25, 435 691, 530350

Abstract:

The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of N,N-dimethyl-3-keto-3-(2-thienyl)-1-ketopropanamine to (S)—N,N-dimethyl-3-hydroxy-3-(2- thienyl)-1-propanamine.

US Patent:

8574876, Nov 5, 2013

Filed:

Mar 30, 2012

Appl. No.:

13/436506

Inventors:

Benjamin Mijts - Belmont CA, US
Sheela Muley - Fremont CA, US
Jack Liang - San Mateo CA, US
Lisa M. Newman - San Jose CA, US
Xiyun Zhang - Fremont CA, US
James Lalonde - Palo Alto CA, US
Michael D. Clay - Menlo Park CA, US
Jun Zhu - Shanghai, CN
John M. Gruber - El Dorado Hills CA, US
Jeffrey Colbeck - Menlo Park CA, US
Jagadeesh Mavinahalli - Singapore, SG
Roger Sheldon - Hoog Keppel, NL

Assignee:

Codexis, Inc. - Redwood City CA

International Classification:

C12P 7/62
C12P 13/22

US Classification:

435135, 435108

Abstract:

The present disclosure provides substantially enantiomerically pure heterobicyclic compounds of the following structural formulas,.