Joseph Chappel Chappell

US Patent:

6468772, Oct 22, 2002

Filed:

Sep 17, 1999

Appl. No.:

09/398395

Inventors:

Joseph Chappell - Lexington KY
Kathleen R. Manna - Georgetown IN
Joseph P. Noel - San Diego CA
Courtney M. Starks - La Jolla CA

Assignee:

The Salk Institute for Biological Studies - San Diego CA
The University of Kentucky Research Foundation - Lexington KY

International Classification:

C12N 900

US Classification:

435183, 435 4, 435232, 435468, 800283, 800284, 800285, 800289, 536 232

Abstract:

Novel polypeptides and the corresponding nucleic acids encoding such polypeptides are disclosed herein. The invention provides methods of making modified polypeptides by altering one or more amino acid residues involved in the active site of a preselected polypeptide.

US Patent:

6495354, Dec 17, 2002

Filed:

Jun 22, 2001

Appl. No.:

09/887586

Inventors:

Joseph Chappell - Lexington KY
Kathleen R. Manna - Georgetown IN
Joseph P. Noel - San Diego CA
Courtney M. Starks - La Jolla CA

Assignee:

University of Kentucky Research Foundation - Lexington KY
The Salk Institute for Biological Studies - San Diego CA

International Classification:

C12N 900

US Classification:

435183, 435 4, 435232, 435468, 800283, 800284, 800285, 800289, 536 232

Abstract:

Novel synthases and the corresponding nucleic acids encoding such synthases are disclosed herein. Such synthases possess an active site pocket that includes key amino acid residues that are modified to generate desired terpenoid reaction intermediates and products. Synthase modifications are designed based on, e. g. , the three-dimensional coordinates of tobacco 5-epi-aristolochene synthase with or without a substrate bound in the active site.

US Patent:

6559297, May 6, 2003

Filed:

Jun 29, 2001

Appl. No.:

09/895752

Inventors:

Joseph Chappell - Lexington KY
Kathleen R. Manna - Georgetown IN
Joseph P. Noel - San Diego CA
Courtney M. Starks - La Jolla CA

Assignee:

The Salk Institute for Biological Studies - La Jolla CA
The University of Kentucky Research Foundation - Lexington KY

International Classification:

C12N 900

US Classification:

536 231, 435 4, 435232, 4352521, 435468, 435325, 435410, 536 232, 536 331, 800283, 800284, 800285, 800289

Abstract:

Novel synthases and the corresponding nucleic acids encoding such synthases are disclosed herein. Such synthases possess an active site pocket that includes key amino acid residues that are modified to generate desired terpenoid reaction intermediates and products. Synthase modifications are designed based on, e. g. , the three-dimensional coordinates of tobacco 5-epi-aristolochene synthase, with or without a substrate bound in the active site.

US Patent:

6569656, May 27, 2003

Filed:

Jul 11, 2001

Appl. No.:

09/903012

Inventors:

Joseph Chappell - Lexington KY
Kathleen R. Manna - Georgetown IN
Joseph P. Noel - San Diego CA
Courtney M. Starks - La Jolla CA

Assignee:

The University of Kentucky Research Foundation - Lexington KY
The Salk Institute for Biological Studies - La Jolla CA

International Classification:

C12N 900

US Classification:

435183, 435 4, 435232, 435468, 536 232, 536 111, 585355, 524 9

Abstract:

Novel synthases and the corresponding nucleic acids encoding such synthases are disclosed herein. Such synthases possess an active site pocket that includes key amino acid residues that are modified to generate desired terpenoid reaction intermediates and products. Synthase modifications are designed based on, e. g. , the three-dimensional coordinates of tobacco 5-epi-aristolochene synthase, with or without a substrate bound in the active site.

US Patent:

6605764, Aug 12, 2003

Filed:

Nov 5, 1999

Appl. No.:

09/435380

Inventors:

Joseph Chappell - Lexington KY
Shaohui Yin - Ardmore OK
Catherine Cornett - Florence KY

Assignee:

University of Kentucky Research Foundation - Lexington KY

International Classification:

A01H 500

US Classification:

8003173, 435419, 800298, 800317

Abstract:

A tobacco epi-5-aristolochene synthase transcriptional regulatory element functional in plants, plant tissue and in plant cells for pathogen inducible gene expression and a method for increasing the transcriptional expression of downstream genetic information in plants, plant tissue and plant cells are disclosed.

US Patent:

6645762, Nov 11, 2003

Filed:

Jul 6, 2001

Appl. No.:

09/900797

Inventors:

Joseph Chappell - Lexington KY
Kathleen R. Manna - Georgetown IN
Joseph P. Noel - San Diego CA
Courtney M. Starks - La Jolla CA

Assignee:

The Salk Institute for Biological Studies - La Jolla CA
The University of Kentucky Research Foundation - Lexington KY

International Classification:

C12N 500

US Classification:

435325, 435183, 435193, 435410, 435468, 800278

Abstract:

Novel synthases and the corresponding nucleic acids encoding such synthases are disclosed herein. Such synthases possess an active site pocket that includes key amino acid residues that are modified to generate desired terpenoid reaction intermediates and products. Synthase modifications are designed based on, e. g. , the three-dimensional coordinates of tobacco 5-epi-aristolochene synthase, with or without a substrate bound in the active site.

US Patent:

6890752, May 10, 2005

Filed:

Jun 28, 2001

Appl. No.:

09/893820

Inventors:

Joseph Chappell - Lexington KY, US
Kathleen R. Manna - Georgetown IN, US
Joseph P. Noel - San Diego CA, US
Courtney M. Starks - La Jolla CA, US

Assignee:

The University of Kentucky Research Foundation - Lexington KY
The Salk Institute for Biological Studies - San Diego CA

International Classification:

C12N005/00
C12N009/00
C12N009/10

US Classification:

435325, 435183, 435193, 435410, 435468, 800278

Abstract:

Novel synthases and the corresponding nucleic acids encoding such synthases are disclosed herein. Such synthases possess an active site pocket that includes key amino acid residues that are modified to generate desired terpenoid reaction intermediates and products. Synthase modifications are designed based on, e. g. , the three-dimensional coordinates of tobacco 5-epi-aristolochene synthase. with or without a substrate bound in the active site.

US Patent:

7186891, Mar 6, 2007

Filed:

Feb 28, 2000

Appl. No.:

09/514513

Inventors:

Joseph Chappell - Lexington KY, US
Kyoungwhan Back - Kwangju, KR

Assignee:

University of Kentucky, Research Foundation - Lexington KY

International Classification:

A01H 5/00
C12N 15/82

US Classification:

800298, 435419

Abstract:

The invention features plant cells and plants that include a nucleic acid molecule encoding a chimeric isoprenoid synthase polypeptide including an asymmetrically positioned homologous domain. The chimeric isoprenoid synthases of the invention catalyze the production of isoprenoid reaction products that are not produced when the asymmetrically positioned homologous domain is positioned at its naturally-occurring site in an isoprenoid synthase polypeptide.