Julie G Mitchell

US Patent:

7416875, Aug 26, 2008

Filed:

Jun 16, 2006

Appl. No.:

11/454379

Inventors:

Ronald T. Raines - Madison WI, US
Julie C. Mitchell - Madison WI, US
Thomas J. Rutkoski - Madison WI, US

Assignee:

Wisconsin Alumni Research Foundation - Madison WI

International Classification:

C12N 9/16
C07K 14/00
C07H 21/00
C12N 5/10
C12N 1/21
C12N 15/00
C12P 21/00
C12Q 1/44

US Classification:

435196, 435 19, 4353201, 435 691, 435325, 4352523, 530350, 536 232

Abstract:

This invention relates to altered forms of members of the RNase A superfamily. An RNase A can be modified to be cytotoxic by altering its amino acid sequence so that it is not bound easily by the ribonuclease inhibitor while still retaining catalytic properties. While earlier work had identified some modifications to RNase A that would result in cytotoxicity, the use of the FADE algorithm for molecular interaction analysis has led to several other locations that were candidates for modification. Some of those modifications did result in RNase A variants with increase cytotoxicity.

US Patent:

7977079, Jul 12, 2011

Filed:

Jul 22, 2008

Appl. No.:

12/177229

Inventors:

Ronald T. Raines - Madison WI, US
Julie C. Mitchell - Madison WI, US
Thomas J. Rutkoski - Madison WI, US

Assignee:

Wisconsin Alumni Research Foundation - Madison WI

International Classification:

C12N 15/00
C12N 5/10
C12N 1/21
C07H 21/00
C12N 9/16
C12P 21/00

US Classification:

435196, 4353201, 4352523, 435325, 435 691, 536 232

Abstract:

This invention relates to altered forms of members of the RNase A superfamily. An RNase A can be modified to be cytotoxic by altering its amino acid sequence so that it is not bound easily by the ribonuclease inhibitor while still retaining catalytic properties. While earlier work had identified some modifications to RNase A that would result in cytotoxicity, the use of the FADE algorithm for molecular interaction analysis has led to several other locations that were candidates for modification. Some of those modifications did result in RNase A variants with increase cytotoxicity.

US Patent:

8247190, Aug 21, 2012

Filed:

Jul 11, 2011

Appl. No.:

13/180359

Inventors:

Ronald T. Raines - Madison WI, US
Julie C. Mitchell - Madison WI, US
Thomas J. Rutkoski - Madison WI, US

Assignee:

Wisconsin Alumni Research Foundation - Madison WI

International Classification:

C12Q 1/44
A61K 38/46
C12N 9/16
C07K 14/00

US Classification:

435 19, 435196, 530350, 424 946

Abstract:

This invention relates to altered forms of members of the RNase A superfamily. An RNase A can be modified to be cytotoxic by altering its amino acid sequence so that it is not bound easily by the ribonuclease inhibitor while still retaining catalytic properties. While earlier work had identified some modifications to RNase A that would result in cytotoxicity, the use of the FADE algorithm for molecular interaction analysis has led to several other locations that were candidates for modification. Some of those modifications did result in RNase A variants with increase cytotoxicity.

US Patent:

8524480, Sep 3, 2013

Filed:

Jul 26, 2012

Appl. No.:

13/559063

Inventors:

Ronald T. Raines - Madison WI, US
Julie C. Mitchell - Madison WI, US
Thomas J. Rutkoski - Madison WI, US

Assignee:

Wisconsin Alumni Research Foundation - Madison WI

International Classification:

C12N 9/16
C07K 14/00
C12Q 1/44
C12P 21/00

US Classification:

435196, 435 19, 435 691, 530350

Abstract:

This invention relates to altered forms of members of the RNase A superfamily. An RNase A can be modified to be cytotoxic by altering its amino acid sequence so that it is not bound easily by the ribonuclease inhibitor while still retaining catalytic properties. While earlier work had identified some modifications to RNase A that would result in cytotoxicity, the use of the FADE algorithm for molecular interaction analysis has led to several other locations that were candidates for modification. Some of those modifications did result in RNase A variants with increase cytotoxicity.

US Patent:

20140004594, Jan 2, 2014

Filed:

Aug 30, 2013

Appl. No.:

14/014952

Inventors:

Julie C. Mitchell - Madison WI, US
Thomas J. Rutkoski - Madison WI, US

Assignee:

Wisconsin Alumni Research Foundation - Madison WI

International Classification:

C12N 9/22

US Classification:

435199

Abstract:

This invention relates to altered forms of members of the RNase A superfamily. An RNase A can be modified to be cytotoxic by altering its amino acid sequence so that it is not bound easily by the ribonuclease inhibitor while still retaining catalytic properties. While earlier work had identified some modifications to RNase A that would result in cytotoxicity, the use of the FADE algorithm for molecular interaction analysis has led to several other locations that were candidates for modification. Some of those modifications did result in RNase A variants with increase cytotoxicity.

US Patent:

20140335593, Nov 13, 2014

Filed:

Jul 18, 2014

Appl. No.:

14/335386

Inventors:

- Madison WI, US
Julie C. Mitchell - Madison WI, US
Thomas J. Rutkoski - Madison WI, US

International Classification:

C12N 9/22

US Classification:

435199

Abstract:

This invention relates to altered forms of members of the RNase A superfamily. An RNase A can be modified to be cytotoxic by altering its amino acid sequence so that it is not bound easily by the ribonuclease inhibitor while still retaining catalytic properties. While earlier work had identified some modifications to RNase A that would result in cytotoxicity, the use of the FADE algorithm for molecular interaction analysis has led to several other locations that were candidates for modification. Some of those modifications did result in RNase A variants with increase cytotoxicity.