Laura Segatori

US Patent:

20070243583, Oct 18, 2007

Filed:

Apr 5, 2007

Appl. No.:

11/697114

Inventors:

George Georgiou - Austin TX, US
Laura Segatori - Houston TX, US

International Classification:

C12N 9/90
C12P 21/06
C07H 21/04
C12N 1/21
C12N 15/74

US Classification:

435069100, 435488000, 435252330, 435233000, 536023200

Abstract:

The present invention provides artificial enzymes comprising, e.g., an N-terminal domain derived from FkpA that allows for dimerization and provides a substrate binding region, and a C-terminal thioredoxin domain derived from DsbA. Similar to DsbC, such de novo designed chimeric (hybrid) FkpA-DsbA enzymes function, as disulfide reductases, oxidases, or isomerases, and chaperones in vivo and in vitro, despite lacking similarity toDsbC-related polypeptide sequence.