Lawrence Restaino

US Patent:

6416970, Jul 9, 2002

Filed:

Sep 19, 2000

Appl. No.:

09/646528

Inventors:

Günter Schabert - Goldach, CH
Lawrence Restaino - Chicago IL

Assignee:

Biosynth AG - Staad

International Classification:

C12Q 104

US Classification:

435 34, 435 4, 435968, 435975

Abstract:

The invention provides compounds of formula (I) in which R , R , R , R and R are independently selected from hydrogen and chromogenic substituents, and X is selected from the group consisting of hydroxyl; OR wherein R is selected from the group consisting of C -C alkyl; and O Me wherein Me is a cation derived from an organic or inorganic base. A preferred species of formula (I) is 4-Methylumbelliferyl myo-inositol-1-phosphate and salts thereof with an organic or inorganic base. The invention also provides fluorogenic methods for detecting various pathogenic bacteria, e. g. , Listeria, Staphylococcus and Clostridium species, using substrates containing at least one formula (I) compound. A kit for detecting a phosphatidyl-inositol-specific phospholipase C enzyme as an indication of bacterial activity is disclosed.

US Patent:

6660494, Dec 9, 2003

Filed:

Dec 19, 2001

Appl. No.:

10/021271

Inventors:

Günter Schabert - Goldach, CH
Lawrence Restaino - Chicago IL
Elon W. Frampton - DeKalb IL

Assignee:

Biosynth AG

International Classification:

C12Q 104

US Classification:

435 34, 435 29, 435 18, 435 21, 435 32, 43525231, 4352533, 435975, 548414

Abstract:

Chromogenic 3-Indoxyl choline phosphate compounds of formula (I): wherein R is selected from the group consisting of hydrogen and C alkyl, such as methyl, ethyl, propyl and butyl while R , R , R , and R are independently selected from the group consisting of hydrogen, halogen, cyano, nitro, carboxy, amino, amino substituted with one or two C alkyl groups, aminomethyl, hydroxy, C alkoxy, carboxyalkyl, and sulphonyl. These compounds are capable of being cleaved by lecithinase C leading to products which are calorimetrically detectable. The invention provides safe and sensitive detection of potentially pathogenic bacterial activity of such microbes as and others in material which may contain such activity typically including physiological samples, goods for consumption, such as food and beverages, and any other potentially infected objects or articles.