Lin C Liu

US Patent:

6777209, Aug 17, 2004

Filed:

Mar 6, 2002

Appl. No.:

10/018878

Inventors:

David Martin Anderson - Rockville MD
Lin Liu - Rockville MD
Sergey Podkovyrov - Gaithersburg MD
Baomin Wang - Rockville MD

Assignee:

SmithKline Beecham Corporation - Philadelphia PA

International Classification:

C12N 120

US Classification:

435 87, 4353201, 435 691, 435325, 4352523, 435194, 435189, 435227, 43525233, 536 232, 530350

Abstract:

Novel DNA constructs and host cells comprising the same are disclosed. DNA constructs comprise a transcription unit (e. g. operon) comprising DNA sequences encoding for ribonucleotide reductase and thioredoxin or a uridine kinase gene and/or a dCTP deaminase gene. In preferred embodiments the constructs comprising DNA sequences encoding for ribonucleotide reductase and thioredoxin further comprise DNA sequences encoding for thymidylate synthase and/or transcription units comprising sequences encoding for uridine kinase preferably together with dCTP deaminase. In particularly preferred embodiments, the host cells comprise constructs having all of the above characteristics wherein the host cell displays repressed or no uracil DNA glycosylase activity. This may be achieved by removal of the host cell ung gene. Use of host cells in the manufacture of pyrimidine deoxyribonucleotides e. g.

US Patent:

6780628, Aug 24, 2004

Filed:

Dec 8, 2000

Appl. No.:

09/731971

Inventors:

David M. Anderson - Rockville MD
Lin Liu - Rockville MD
Humg-Yu Hsiao - Rockville MD
Douglas W. Fodge - Derwood MD

Assignee:

Chemgen Corporation - Gaithersburg MD

International Classification:

A23L 131

US Classification:

435198, 435 4, 435 6, 435183, 435195, 426 2, 426 56, 426531

Abstract:

Enzymes of a particular class, characterized by the ability to cleave a linkage that effects release of a cell-surface protein or carbohydrate, which does not contain an anti-infection agent, display significant anti-infectious activity. Upon oral administration, these enzymes are effective, for example, in the treatment of digestive tract infections in humans and in animals. In the latter, there are benefits of significantly improved growth rate, feed efficiency, and overall health.

US Patent:

7387893, Jun 17, 2008

Filed:

Aug 9, 2004

Appl. No.:

10/914529

Inventors:

David Martin Anderson - Rockville MD, US
Lin Liu - Rockville MD, US
Sergey Podkovyrov - Gaithersburg MD, US
Baomin Wang - Rockville MD, US

Assignee:

SmithKline Beecham Corporation - Philadelphia PA

International Classification:

C12N 15/00
C12N 1/20
C12N 5/10
C07H 21/00
C12N 9/02
C12N 9/12
C12N 9/78
C12Q 1/26
C12P 21/06
C07K 14/00

US Classification:

4353201, 4352523, 435325, 43525233, 435189, 435 25, 435 691, 435194, 435227, 536 232, 530350

Abstract:

Novel DNA constructs and host cells comprising the same are disclosed. DNA constructs comprise a transcription unit (e. g. operon) comprising DNA sequences encoding for ribonucleotide reductase and thioredoxin or a uridine kinase gene and/or a dCTP deaminase gene. In preferred embodiments the constructs comprising DNA sequences encoding for ribonucleotide reductase and thioredoxin further comprise DNA sequences encoding for thymidylate synthase and/or transcription units comprising sequences encoding for uridine kinase preferably together with dCTP deaminase. In particularly preferred embodiments, the host cells comprise constructs having all of the above characteristics wherein the host cell displays repressed or no uracil DNA glycosylase activity. This may be achieved by removal of the host cell ung gene. Use of host cells in the manufacture of pyrimidine deoxyribonucleotides e. g.

US Patent:

7723091, May 25, 2010

Filed:

May 28, 2004

Appl. No.:

10/855416

Inventors:

David M. Anderson - Rockville MD, US
Lin Liu - Rockville MD, US
Humg-Yu Hsiao - Rockville MD, US
Douglas W. Fodge - Derwood MD, US

Assignee:

Chemgen Corporation - Gaithersburg MD

International Classification:

C12N 9/24
C12N 9/00
C12Q 1/00
C12Q 1/68
A23L 1/31
C07H 21/04

US Classification:

435200, 426 58, 435 4, 435 6, 435 691, 435183, 530350, 536 232

Abstract:

Enzymes of a particular class, characterized by the ability to cleave a linkage that effects release of a cell-surface protein or carbohydrate, which does not contain an anti-infection agent, display significant anti-infectious activity. Upon oral administration, these enzymes are effective, for example, in the treatment of digestive tract infections in humans and in animals. In the latter, there are benefits of significantly improved growth rate, feed efficiency, and overall health.

US Patent:

7914782, Mar 29, 2011

Filed:

Dec 14, 2006

Appl. No.:

11/610572

Inventors:

David M. Anderson - Rockville MD, US
Humg-Yu Hsiao - Rockville MD, US
Lin Liu - Rockville MD, US

Assignee:

Chemgen Corporation - Gaithersburg MD

International Classification:

A61K 38/47

US Classification:

424 9461

Abstract:

Compositions suitable for oral administration to an animal comprising at least one immune stress-reducing enzyme in an amount effective to decrease the level of positive acute phase protein in an animal, increase the level of negative acute phase protein in an animal, and/or improve animal growth performance is provided, as are methods using such compositions. The compositions include animal feed compositions, liquid compositions other than animal feed, and solid compositions other than animal feed.

US Patent:

8263384, Sep 11, 2012

Filed:

Feb 5, 2007

Appl. No.:

12/278227

Inventors:

David Martin Anderson - Gaithersburg MD, US
Andrew John Collis - Ulverston, GB
Lin Liu - Gaithersburg MD, US
Sergey Podkovyrov - Gaithersburg MD, US
Christopher Preston - Ulverston, GB

Assignee:

Glaxo Group Limited - Greenford, Middlesex

International Classification:

C12N 1/21
C12P 19/38

US Classification:

4352523, 435189, 4353201, 435 87

Abstract:

Novel organisms, including DNA construct host cell combinations, are disclosed. The organisms comprise a transcription unit (e. g. operon) comprising DNA sequences encoding for enzymes which promote the supply of single carbon units for the conversion of dUMP to dTMP. Examples include: dihydrofolate reductase genes e. g. T4 frd; Serine Hydroxymethyltransferase genes e. g. glyA; 3-phosphoglycerate dehydrogenase genes e. g. serA; and THF synthase genes e. g. ADE3. The organisms are used in a biological method of producing thymidine with significantly reduced levels of uridine.

US Patent:

20110171344, Jul 14, 2011

Filed:

Mar 25, 2011

Appl. No.:

13/072109

Inventors:

DAVID M. ANDERSON - ROCKVILLE MD, US
HUMG-YU HSIAO - ROCKVILLE MD, US
LIN LIU - ROCKVILLE MD, US

International Classification:

A23K 1/165

US Classification:

426 2, 426 61

Abstract:

Compositions suitable for oral administration to an animal comprising at least one immune stress-reducing enzyme in an amount effective to decrease the level of positive acute phase protein in an animal, increase the level of negative acute phase protein in an animal, and/or improve animal growth performance is provided, as are methods using such compositions. The compositions include animal feed compositions, liquid compositions other than animal feed, and solid compositions other than animal feed.

US Patent:

20110171345, Jul 14, 2011

Filed:

Mar 25, 2011

Appl. No.:

13/072123

Inventors:

David M. Anderson - Rockville MD, US
Humg-Yu Hsiao - Rockville MD, US
Lin Liu - Rockville MD, US

International Classification:

A23K 1/165

US Classification:

426 2, 426 61

Abstract:

Compositions suitable for oral administration to an animal comprising at least one immune stress-reducing enzyme in an amount effective to decrease the level of positive acute phase protein in an animal, increase the level of negative acute phase protein in an animal, and/or improve animal growth performance is provided, as are methods using such compositions. The compositions include animal feed compositions, liquid compositions other than animal feed, and solid compositions other than animal feed.