Lin T Lu

US Patent:

20030005891, Jan 9, 2003

Filed:

Jul 9, 2001

Appl. No.:

09/900548

Inventors:

Lin Lu - Wayne PA, US

International Classification:

A01K023/00

US Classification:

119/867000

Abstract:

A sheet for collecting solid dog waste while the dog is being walked outside including a square plastic film with rounded corners, a string going through the four folded edges, and a circular paper stuck in the center of the plastic film. The solid dog waste will be eliminated on the sheet, so the ground or lawn will remain clean. Using this sheet, dog owners will keep their hands away from touching the solid dog waste. They will not feel dirty anymore. The product is low in cost; disposal is easy.

US Patent:

20200299650, Sep 24, 2020

Filed:

Mar 31, 2017

Appl. No.:

16/088743

Inventors:

- Philadelphia PA, US
Younghoon OH - Bryn Mawr PA, US
Lin LU - Wayne PA, US
John Fraser WRIGHT - Princeton NJ, US

Assignee:

SPARK THERAPEUTICS, INC. - Philadelphia PA

International Classification:

C12N 7/00
C12N 1/06
B01D 15/36
B01D 15/34
B01D 61/14
B01D 15/38

Abstract:

In accordance with the invention, provided herein are methods for purifying recombinant adeno-associated (rAAV) vector particles.

US Patent:

20200165632, May 28, 2020

Filed:

Jun 6, 2018

Appl. No.:

16/619898

Inventors:

- Philadelphia PA, US
Lin LU - Wayne PA, US
John Fraser WRIGHT - Princeton NJ, US

Assignee:

Spark Therapeutics, Inc. - Philadelphia PA

International Classification:

C12N 15/87
C12N 5/073

Abstract:

Provided are compositions and methods of transducing/transfecting cells with a molecule, such as a nucleic acid (e.g., plasmid), at high efficiency. High efficiency transduced/transfected cells can, when transduced with a nucleic acid that encodes a protein or comprises a sequence that is transcribed into a transcript of interest, produce high amounts of protein and/or transcript. High efficiency transduced/transfected cells can, when transduced with plasmids comprising (i) nucleic acids encoding AAV packaging proteins and/or nucleic acids encoding helper proteins; and (ii) a transgene that encodes a protein or is transcribed into a transcript of interest; produce high amounts of recombinant rAAV vector.

US Patent:

20190292561, Sep 26, 2019

Filed:

Dec 1, 2016

Appl. No.:

15/780542

Inventors:

- Philadelphia PA, US
Lin LU - Wayne PA, US
John Fraser WRIGHT - Princeton NJ, US

Assignee:

SPARK THERAPEUTICS, INC. - Philadelphia PA

International Classification:

C12N 15/86
C12N 5/00
C12N 5/073
C12N 5/071

Abstract:

Methods and compositions for transfecting cells with plasmids are disclosed. In certain embodiments, methods and compositions are disclosed in which transfection efficiency is significantly increased by contacting the cells being transduced with polyethyleneimine (PEI) that is free of nucleic acid during the transfection process. Therapeutically useful adeno-associated viral vectors generated according to the disclosed methods and compositions are also disclosed.