Lishan Zhao

US Patent:

6943001, Sep 13, 2005

Filed:

Oct 10, 2002

Appl. No.:

10/272490

Inventors:

Lishan Zhao - Carlsbad CA, US
Eric J. Mathur - Carlsbad CA, US
David Weiner - Del Mar CA, US
Toby Richardson - San Diego CA, US
Aileen Milan - San Diego CA, US
Mark J. Burk - San Diego CA, US
Bin Han - San Diego CA, US
Jay M. Short - Rancho Santa Fe CA, US

Assignee:

Diversa Corporation - San Diego CA

International Classification:

C12N009/14
C12N015/54
C12Q001/34
C07K002/04

US Classification:

435195, 435 18, 4352523, 435325, 435410, 4352541, 4352551, 4353201, 536 232, 536 2433

Abstract:

The invention is directed to polypeptides having epoxide hydrolase activity, polynucleotides encoding the polypeptides, antibodies that bind to these polypeptides, and methods for making and using these polynucleotides and polypeptides. The epoxide hydrolases are used to catalyze the hydrolysis of epoxides and arene oxides to their corresponding diols.

US Patent:

6979733, Dec 27, 2005

Filed:

Aug 5, 2002

Appl. No.:

10/214473

Inventors:

Lishan Zhao - Carlsbad CA, US
Eric J. Mathur - Carlsbad CA, US
David Weiner - Del Mar CA, US
Toby Richardson - San Diego CA, US
Aileen Milan - San Diego CA, US
Mark J. Burk - San Diego CA, US
Bin Han - San Diego CA, US
Jay M. Short - Rancho Santa Fe CA, US

Assignee:

Diversa Corporation - San Diego CA

International Classification:

C12N015/55
C12Q001/68

US Classification:

536 232, 536 243, 536 2433, 435 3, 435195, 4353201, 4352523, 4352541, 4352551, 435325, 435348, 435410

Abstract:

The invention is directed to polypeptides having epoxide hydrolase activity, polynucleotides encoding the polypeptides, antibodies that bind to these polypeptides, and methods for making and using these polynucleotides and polypeptides. The epoxide hydrolases are used to catalyze the hydrolysis of epoxides and arene oxides to their corresponding diols.

US Patent:

7572607, Aug 11, 2009

Filed:

Apr 26, 2005

Appl. No.:

11/114922

Inventors:

Paula M. Hicks - Bend OR, US
Sara C. McFarlan - St. Paul MN, US
Timothy W. Abraham - Minnetonka MN, US
Douglas C. Cameron - Plymouth MN, US
James R. Millis - Plymouth MN, US
John Rosazza - Iowa City IA, US
Lishan Zhao - Carlsbad CA, US
David Paul Weiner - Del Mar CA, US

Assignee:

Cargill, Incorporated - Wayzata MN

International Classification:

C12P 13/22
C12N 1/21

US Classification:

435121, 435108, 435193, 435 691, 435325, 4353201, 4352523, 536 232

Abstract:

Methods and compositions that can be used to make monatin from glucose, tryptophan, indole-3-lactic acid, indole-3-pyruvate, and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid, are provided. Methods are also disclosed for producing the indole-3-pyruvate and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid intermediates. Compositions provided include nucleic acid molecules, polypeptides, chemical structures, and cells. Methods include in vitro and in vivo processes, and the in vitro methods include chemical reactions.

US Patent:

7582455, Sep 1, 2009

Filed:

Apr 26, 2006

Appl. No.:

11/411229

Inventors:

Brian J. Brazeau - Oskaloosa IA, US
Ellen Burke - San Diego CA, US
Mervyn DeSouza - Plymouth MN, US
Steven J. Gort - Brooklyn Center MN, US
Paula M. Hicks - Eden Prairie MN, US
Sherry R. Kollmann - Maple Grove MN, US
Peter Luginbuhl - San Diego CA, US
Sara C. McFarlan - St. Paul MN, US
Toby Richardson - San Diego CA, US
Fernando A. Sanchez-Riera - Eden Prairie MN, US
Christopher Solheid - Minneapolis MN, US
David Weiner - Del Mar CA, US
Lishan Zhao - Carlsbad CA, US

Assignee:

Cargill, Incorporated - Wayzata MN

International Classification:

C12P 17/10

US Classification:

435121

Abstract:

Monatin and certain stereoisomers of monatin, such as R,R monatin and S,R monatin, as well as salts thereof, are produced using polypeptides and biosynthetic pathways. These polypeptides and biosynthetic pathways are also useful in the production of R-2-hydroxy-2-(indoly-3-ylmethyl)-4-keto glutaric acid, an intermediate that is formed in certain monatin synthesis pathways, including some biosynthetic pathways.

US Patent:

7981844, Jul 19, 2011

Filed:

Jun 21, 2002

Appl. No.:

10/178938

Inventors:

David Weiner - Del Mar CA, US
Tim Hitchman - San Diego CA, US
Lishan Zhao - Carlsbad CA, US
Mark J. Burk - San Diego CA, US
Grace Desantis - San Diego CA, US
Sarah Richardson Hanson - San Diego CA, US
Aileen Milan - San Diego CA, US
Toby Richardson - San Diego CA, US
Patti Kretz - San Marcos CA, US
William Greenberg - San Diego CA, US

Assignee:

Verenium Corporation - San Diego CA

International Classification:

C40B 30/08
C12P 21/06
C12N 9/00

US Classification:

506 11, 435 691, 435183

Abstract:

The invention provides biocatalytic methods for the manufacture of pure single enantiomer compounds. This invention provides methods of screening for enzymes which are highly enantioselective or enzymes that can provide any desired stereoisomer of a compound. The invention provides the use of single enantiomer substrates in performing a growth screen of a clonal library to identify highly stereoselective enzymes. In one aspect, methods for screening and identification of enzymes, e. g. , transaminases, nitrilases, aldolases, epoxide hydrolases are provided. Methods for the production and screening of gene libraries generated from nucleic acids isolated from more than one organism for enzyme, e. g. , transaminase, activities are also provided.

US Patent:

8101393, Jan 24, 2012

Filed:

Dec 8, 2006

Appl. No.:

12/278958

Inventors:

Kevin A. Gray - San Diego CA, US
Lishan Zhao - Emeryville CA, US
Michelle H. Cayouette - San Diego CA, US

Assignee:

BP Corporation North America Inc. - Houston TX

International Classification:

C12N 9/42
C12N 15/00
C12P 7/10
C12P 21/06
C07H 21/04
C07H 5/06
C07K 1/00
A61K 38/47
A23L 2/00
A23L 1/202
A23L 1/30

US Classification:

435209, 435165, 435 691, 4353201, 536 232, 536 553, 530350, 424 9461, 426590, 426 64, 426648

Abstract:

The invention provides polypeptides having any cellulolytic activity, e. g. , a cellulase activity, a endoglucanase, a cellobiohydrolase, a beta-glucosidase, a xylanase, a mannanse, a β-xylosidase, an arabinofuranosidase, and/or an oligomerase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having any cellulolytic activity, e. g. , a cellulase activity, e. g. , endoglucanase, cellobiohydrolase, beta-glucosidase, xylanase, mannanse, β-xylosidase, arabinofuranosidase, and/or oligomerase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. In one aspect, the invention provides polypeptides having an oligomerase activity, e. g. , enzymes that convert recalcitrant soluble oligomers to fermentable sugars in the saccharification of biomass.

US Patent:

8236512, Aug 7, 2012

Filed:

Feb 1, 2012

Appl. No.:

13/363588

Inventors:

Lishan Zhao - Emeryville CA, US
Lan Xu - San Diego CA, US
Patrick Westfall - Novato CA, US
Andrew Main - Berkeley CA, US

Assignee:

Amyris, Inc. - Emeryville CA

International Classification:

G01N 33/53
C12P 1/00

US Classification:

435 76

Abstract:

The present disclosure relates to methods of developing terpene synthase variants through engineered host cells. Particularly, the disclosure provides methods of developing terpene synthase variants with improved in vivo performance that are useful in the commercial production of terpene products. Further encompassed in the present disclosure are superior terpene synthase variants and host cells comprising such terpene synthase variants.

US Patent:

8372989, Feb 12, 2013

Filed:

Nov 3, 2004

Appl. No.:

10/979821

Inventors:

Timothy W. Abraham - Minnetonka MN, US
Douglas C. Cameron - Plymouth MN, US
Paula M. Hicks - Eden Prairie MN, US
Sara C. McFarlan - St. Paul MN, US
John Rosazza - Iowa City IA, US
Lishan Zhao - Carlsbad CA, US
David P. Weiner - Del Mar CA, US

Assignee:

Cargill, Incorporated - Wayzata MN

International Classification:

C07D 209/18
C12P 13/22
C12P 21/06
C07H 21/04
C12N 9/10

US Classification:

548495, 435108, 435 691, 435193, 4353201, 4352523, 435325, 536 232

Abstract:

Methods and compositions that can be used to make monatin from glucose, tryptophan, indole-3-lactic acid, indole-3-pyruvate, and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid, are provided. Methods are also disclosed for producing the indole-3-pyruvate and 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid intermediates. Compositions provided include nucleic acid molecules, polypeptides, chemical structures, and cells. Methods include in vitro and in vivo processes, and the in vitro methods include chemical reactions.