Manjit Singh

US Patent:

20210254096, Aug 19, 2021

Filed:

Apr 29, 2021

Appl. No.:

17/244603

Inventors:

- Johnston IA, US
UTE BAUMANN - GLEN OSMOND, AU
ANDREW MARK CIGAN - MADISON WI, US
MANJIT SINGH - JOHNSTON IA, US
ELISE TUCKER - CLAPHAM, AU
RYAN WHITFORD - EASTWOOD, AU

Assignee:

PIONEER HI-BRED INTERNATIONAL, INC. - JOHNSTON IA

International Classification:

C12N 15/82

Abstract:

Compositions and methods are capable of modulating male fertility in a plant. Compositions comprise polynucleotides and polypeptides, and fragments and variants thereof, which modulate male fertility. Expression cassettes comprise a male-fertility polynucleotide, or fragment or variant thereof, operably linked to a promoter, wherein expression of the polynucleotide modulates the male fertility of a plant. The level and/or activity of a polynucleotide that influences male fertility is modulated in a plant or plant part. Regulatory sequences drive expression in a male-tissue-preferred manner and may be targets to downregulate an operably linked gene. Methods to track mutations that induce nuclear recessive male sterility in subsequent selfing and crossing of wheat lines containing the mutations are also provided. Male-sterile plants may be maintained by pollinating with a maintainer plant.

US Patent:

20210222192, Jul 22, 2021

Filed:

Dec 10, 2018

Appl. No.:

16/769019

Inventors:

- JOHNSTON IA, US
ANDREW MARK CIGAN - MADISON WI, US
MARGARET ANNE PALLOTTA - NORWOOD, AU
MANJIT SINGH - JOHNSTON IA, US
RADOSLAW SUCHECKI - PASADENA, AU
NATHAN SPENCER WATSON-HAIGH - NOVAR GARDENS, AU
RYAN WHITFORD - EASTWOOD, AU

Assignee:

PIONEER HI-BRED INTERNATIONAL, INC. - JOHNSTON IA

International Classification:

C12N 15/82
C07K 14/415

Abstract:

Compositions and methods are provided for genome modification of a nucleotide sequence located in or near a male fertility gene of Ms1 or Ms5 in the genome of a plant cell or plant to produce a male-sterile plant. In some examples, the methods and compositions employ a guide RNA/Cas endonuclease system for modifying or altering target sites located in or near a male fertility gene of Ms1 or Ms5 in the genome of a plant cell, plant or seed to produce a male-sterile plant. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification a nucleotide sequence located in or near a male fertility gene of Ms1 or Ms5 in the genome of a plant cell to produce a male-sterile plant. Compositions and methods are also provided for restoring fertility to a Ms1 or Ms5 nucleotide sequence to a male-sterile Ms1 or Ms5 plant produced using the methods and compositions described herein.

US Patent:

20200087678, Mar 19, 2020

Filed:

Nov 27, 2019

Appl. No.:

16/697681

Inventors:

- Johnston IA, US
UTE BAUMANN - GLEN OSMOND, AU
ANDREW MARK CIGAN - MADISON WI, US
MANJIT SINGH - JOHNSTON IA, US
ELISE TUCKER - CLAPHAM, AU
RYAN WHITFORD - EASTWOOD, AU

Assignee:

PIONEER HI-BRED INTERNATIONAL, INC. - JOHNSTON IA

International Classification:

C12N 15/82

Abstract:

Compositions and methods are capable of modulating male fertility in a plant. Compositions comprise polynucleotides and polypeptides, and fragments and variants thereof, which modulate male fertility. Expression cassettes comprise a male-fertility polynucleotide, or fragment or variant thereof, operably linked to a promoter, wherein expression of the polynucleotide modulates the male fertility of a plant. Regulatory sequences drive expression in a male-tissue-preferred manner and may be targets to downregulate an operably-linked gene. Mutations that induce nuclear recessive male sterility in subsequent selfing and crossing of wheat lines containing the mutations may be tracked. Male-sterile plants may be maintained by crossing with a maintainer plant. Hybrids can be produced using the male-sterile plants.

US Patent:

20170298383, Oct 19, 2017

Filed:

Sep 21, 2015

Appl. No.:

15/514195

Inventors:

- Johnston IA, US
UTE BAUMANN - GLEN OSMOND, AU
ANDREW MARK CIGAN - De Forest WI, US
MANJIT SINGH - Johnston IA, US
ELISE TUCKER - CLAPHAM, AU
RYAN WHITFORD - EASTWOOD, AU

International Classification:

C12N 15/82
C07K 14/415
C12N 15/82
C12N 15/82

Abstract:

Compositions and methods are capable of modulating male fertility in a plant. Compositions comprise polynucleotides and polypeptides, and fragments and variants thereof, which modulate male fertility. Expression cassettes comprise a male-fertility polynucleotide, or fragment or variant thereof, operably linked to a promoter, wherein expression of the polynucleotide modulates the male fertility of a plant. The level and/or activity of a polynucleotide that influences male fertility is modulated in a plant or plant part. Regulatory sequences drive expression in a male-tissue-preferred manner and may be targets to downregulate an operably linked gene. Methods to track mutations that induce nuclear recessive male sterility in subsequent selfing and crossing of wheat lines containing the mutations are also provided. Male-sterile plants may be maintained by pollinating with a maintainer plant.