Michael James Tiemeyer

US Patent:

20100297609, Nov 25, 2010

Filed:

Oct 29, 2008

Appl. No.:

12/734102

Inventors:

Robert Lance Wells - Athens GA, US
Ronald C. Orlando - Athens GA, US
Stephen Dalton - Athens GA, US
Kelley W. Moreman - Bishop GA, US
Michael J. Pierce - Athens GA, US
Michael Tiemeyer - Watkinsville GA, US
William S. York - Watkinsville GA, US

Assignee:

University of Georgia Research Foundation, Inc. - Athens GA

International Classification:

C12Q 1/70
C12Q 1/02

US Classification:

435 5, 435 29

Abstract:

The present invention relates to a method of isotopically labeling glycans and in facilitating high throughput quantitative/comparative analysis of glycomic compositions of biological cells. The method is applicable inter alia for identifying differentiated cells and their glycomic characteristics, differentiation conditions, disease and/or therapeutic progression, diagnosing disease states, determining drug activity, establishing manufacturing efficiencies and for determining the half-life of glycans in cells.

US Patent:

50192310, May 28, 1991

Filed:

Feb 16, 1990

Appl. No.:

7/481367

Inventors:

Brian K. Brandley - Alameda CA
Paul G. James - Alameda CA
Michael Tiemeyer - Oakland CA

Assignee:

Glycomed, Incorporated - Alameda CA

International Classification:

C07H 106
C25B 700

US Classification:

2041821

Abstract:

Saccharide in mixtures are separated into groups or individual saccharides and tested for their affinity for particular proteins. The method of the invention is carried out by conjugating saccharides in a mixture with 4-amino-1-naphthalene sulfonic acid (ANSA) to form conjugates. The saccharide/ANSA conjugates are subjected to electrophoretic resolution within gels. The resolved bands of material are electro-blotted onto charged nylon membranes to provide a stable record of the electrophoretic separation. The blots on the nylon membranes are brought into contact with particular proteins in order to determine the binding affinity of these particular proteins to particular saccharides on the nylon membrane. The proteins are preferably bound to labels so that the binding of the proteins to the saccharides can be easily detected.

US Patent:

50873377, Feb 11, 1992

Filed:

Sep 27, 1990

Appl. No.:

7/589441

Inventors:

Brian K. Brandley - Alameda CA
Michael Tiemeyer - Oakland CA
Robert J. Stack - Alameda CA

Assignee:

Glycomed, Inc. - Alameda CA

International Classification:

G01N 2726

US Classification:

2041821

Abstract:

A method of separating mixtures of saccharides into distinct detectable groups is disclosed. In accordance with the method a tri-functional conjugate must first be provided. The tri-functional conjugate is obtained by reacting a mixture of saccharides with moieties (a) capable of providing a charge upon ionization; (b) capable of fluorescing under ultraviolet light; and (c) having a light-activatable azido group thereon. The different functional moieties may all be present on the same moiety and connected directly to the saccharide or may be connected to each other wherein only one of the moieties is connected directly to the saccharide. The tri-functional conjugates are subjected to electrophoretic separation to obtain separate groups of conjugates in the gel. The groups of conjugates are transferred from the gel to the surface of a membrane which is exposed to light for a sufficient time and light frequency to activate the azido-group. The light-activated azido-groups attached to the surface of the membrane.

US Patent:

56483449, Jul 15, 1997

Filed:

Jul 30, 1992

Appl. No.:

7/922328

Inventors:

Brian K. Brandley - Alameda CA
Michael Tiemeyer - Oakland CA
Stuart J. Swiedler - Oakland CA
Margaret Moreland - Berkeley CA
Hans Schweingruber - Mountain View CA
Narasinga Rao - Alameda CA

Assignee:

Glycomed Incorporated - San Diego CA

International Classification:

A01N 4304

US Classification:

514 61

Abstract:

Ligands that bind to human selectin receptors are disclosed. The ligands are formulated with excipient carriers to form compositions which are administered to treated condition such as inflammation. The ligands have the structural formula III ##STR1## or molecules which have hydrogen bond donor groups equivalent to the circled groups with respect to their ability to form hydrogen bonds with a selectin under physiological conditions.

US Patent:

54280257, Jun 27, 1995

Filed:

Mar 2, 1992

Appl. No.:

7/844101

Inventors:

Brian K. Brandley - Alameda CA
Michael Tiemeyer - Oakland CA
Stuart J. Swiedler - Oakland CA
Margaret Moreland - Berkeley CA
Hans Schweingruber - Mountain View CA

Assignee:

Glycomed Incorporated - Alameda CA

International Classification:

A01N 4304
A61K 3173

US Classification:

514 55

Abstract:

Ligands in the form of N-acetyllactosamines which bind to endothelial leukocyte adhesion molecule-1 (ELAM-1) are disclosed. The ligand compounds can be formulated into pharmaceutical compositions and/or assay compositions used to alleviate inflammation and assay for the presence of (qualitative) and amount of (quantitative) ELAM-1 and thereby determine the presence, location and degree of inflammation. The ligands are encompassed by general structural formula I as follows: ##STR1## wherein one of F and G is hydrogen and one is an N-acetyl neuraminic acid residue on the terminal unit and are both hydrogen on any other unit; J is hydrogen, a lactosylceramide or a linking group and K is hydrogen or a fucose residue and n is an integer of from 1 to 10 (preferably 3 or 4) with the proviso that n and K are defined such that at least one K is a fucose residue.

US Patent:

51437120, Sep 1, 1992

Filed:

Jan 7, 1991

Appl. No.:

7/637868

Inventors:

Brian K. Brandley - Alameda CA
Michael Tiemeyer - Oakland CA
Stuart J. Swiedler - Oakland CA
Margaret Moreland - Berkeley CA
Hans Schweingruber - Mountain View CA

Assignee:

Glycomed Incorporated - Alameda CA

International Classification:

A61K 4902
A61K 4900

US Classification:

424 11

Abstract:

Ligands in the form of N-acetyllactosamines which bind to endothelial leukocyte adhesion molecule-1 (ELAM-1) are disclosed. The ligand compounds can be formulated into pharmaceutical compositions and/or assay compositions used to alleviate inflammation and assay for the presence of (qualitative) and amount of (quantitative) ELAM-1 and thereby determine the presence, location and degree of inflammation. The ligands are encompassed by general structural formula I as follows: ##STR1## wherein one of A and B is hydrogen and one is an N-acetyl neuraminic acid residue; D and E are each independently hydrogen or a fucose residue; n is an integer of from 0 to 10 with the proviso that if n is 0, E is a fucose residue; F is hydrogen, a lactosylceramide residue or a linking group; and molecules equivalent to a compound of formula I regarding its ability to bind to an ELAM-1 receptor to the same degree as a compound of formula I.

US Patent:

52119360, May 18, 1993

Filed:

Jul 30, 1990

Appl. No.:

7/559836

Inventors:

Brian K. Brandley - Alameda CA
Michael Tiemeyer - Oakland CA
Stuart J. Swiedler - Oakland CA
Margaret Moreland - Berkeley CA
Hans Schweingruber - Mountain View CA

Assignee:

Glycomed Incorporated - Alameda CA

International Classification:

A61K 4902
A61K 4900

US Classification:

424 11

Abstract:

Ligands in the form of N-acetyllactosamines which bind to endothelial leukocyte adhesion molecule-1 (ELAM-1) are disclosed. The ligand compounds can be formulated into pharmaceutical compositions and/or assay compositions used to alleviate inflammation and assay for the presence of (qualitative) and amount of (quantitative) ELAM-1 and thereby determine the presence, location and degree of inflammation. The ligands are encompassed by general structural formula I as follows: ##STR1## wherein one of F and G is hydrogen and one is an N-acetyl neuraminic acid residue on the terminal unit and are both hydrogen on any other unit; J is hydrogen, a lactosylceramide or a linking group and K is hydrogen or a fucose residue and n is an integer of from 1 to 10 (preferably 3 or 4) with the proviso that n and K are defined such that at least one K is a fucose residue.

US Patent:

50947311, Mar 10, 1992

Filed:

Sep 27, 1990

Appl. No.:

7/589431

Inventors:

Brian K. Brandley - Alameda CA
Paul G. James - Alameda CA
Michael Tiemeyer - Oakland CA

Assignee:

Glycomed, Inc. - Alameda CA

International Classification:

G01N 2726

US Classification:

2041821

Abstract:

Saccharide in mixtures are separated into groups or individual saccharides and tested for their affinity for particular proteins. The method of the invention is carried out by conjugating saccharides in a mixture with charge-generating moieties and moieties capable of fluorescing such as 4-amino-1-naphthalene sulfonic acid (ANSA) to form conjugates. The saccharide conjugates are subjected to electrophoretic resolution within gels. The resolved bands of material can be seen under ultraviolet light and are electro-blotted onto charged nylon membranes to provide a stable record of the electrophoretic separation. The blots (visible under ultraviolet light) on the nylon membranes are brought into contact with particular proteins in order to determine the binding affinity of these particular proteins to particular saccharides on the nylon membrane. The proteins are preferably bound to labels so that the binding of the proteins to the saccharides can be easily detected.