Ning Liu

US Patent:

20100125128, May 20, 2010

Filed:

Nov 9, 2009

Appl. No.:

12/615080

Inventors:

Ning Liu - Fremont CA, US
Katrina Academia - Hercules CA, US
Aran Paulus - San Jose CA, US
Tim Wehr - Albany CA, US
Steve Freeby - Vacaville CA, US

Assignee:

BIO-RAD LABORATORIES, INC. - Hercules CA

International Classification:

C07K 1/14

US Classification:

530344

Abstract:

Phosphorylated peptides are extracted from digests of biological liquids and other peptide mixtures by fractionation on ceramic hydroxyapatite. The ceramic hydroxyapatite is readily usable in a centrifuge, allowing for rapid fractionations of a large number of small volume samples, and accordingly high throughput.

US Patent:

20130288388, Oct 31, 2013

Filed:

Apr 25, 2013

Appl. No.:

13/870710

Inventors:

Ning Liu - Fremont CA, US
Kevin McDonald - Novato CA, US
Aran Paulus - San Jose CA, US
Anton Posch - Grafting, DE

Assignee:

Bio-Rad Laboratories, Inc., LSG - LSD Division - Hercules CA

International Classification:

G01N 33/53

US Classification:

436501

Abstract:

Disclosed herein are methods of protein quantification and normalization using haloalkylated tryptophan fluorescence. Complex protein samples, i.e., samples that each contain 1,000 or more distinct proteins, from diverse sources that do not have common protein profiles are treated with a halo-substituted organic compound (i.e. haloalkane) that reacts with tryptophan residues to form fluorescent products. Irradiation of the samples with ultraviolet light and the detection and quantification of the resultant fluorescent emissions from all proteins in each sample are then used to obtain comparative values for total protein content among the various samples. The values thus obtained are found to be valid indications of comparative total protein content, despite the fact that the tryptophan levels vary widely among the various proteins in any single sample and the samples, due to the diversity of their origins, tend to differ among themselves in the identities and relative amounts of the proteins that they contain. Protein samples are also normalized to correct for differences in sample dilution, sample loading, and protein transfer inconsistencies, by using stain-free detection of total protein in each of the samples, or detection of subsamples within each sample.

US Patent:

20210116369, Apr 22, 2021

Filed:

Oct 22, 2020

Appl. No.:

17/077643

Inventors:

- Hercules CA, US
NING LIU - WALNUT CREEK CA, US
CARL MARLOWE - SAN FRANCISCO CA, US
WILLIAM BRADLEY STRONG - EL CERRITO CA, US

International Classification:

G01N 21/552
G06T 7/00

Abstract:

The invention relates to methods for characterizing the binding interactions between binding partners. The invention pertains to methods comprising contacting a first binding partner with a second binding partner that exhibits fast-off rate binding characteristics with the first binding partner to generate the binding interaction between the first binding partner and the second binding partner, wherein the first binding partner is immobilized onto a substrate that is designed to enhance the fluorescence signal of fluorescent molecules located near the substrate's surface, and wherein the second binding partner is a molecule that emits a fluorescent signal or is conjugated to molecule that emits a fluorescent signal. The interactions between the two binding partners can be analyzed based on multiple transient interactions between the two binding partners.

US Patent:

20190339266, Nov 7, 2019

Filed:

Dec 19, 2017

Appl. No.:

16/475247

Inventors:

- Hercules CA, US
Ning LIU - Walnut Creek CA, US

International Classification:

G01N 33/557
G01N 33/52
G01N 33/68
G01N 21/64
G06T 7/00
G06T 7/13

Abstract:

Methods for characterizing the binding characteristic of at least one protein molecule to a detectably labeled probe are provided.

US Patent:

20190178877, Jun 13, 2019

Filed:

Oct 12, 2018

Appl. No.:

16/158853

Inventors:

- Herculer CA, US
Ning LIU - Fremont CA, US
Kevin McDONALD - Novato CA, US
Aran PAULUS - San Jose CA, US
Anton POSCH - Grafing, DE

International Classification:

G01N 33/53
G16B 99/00
G06T 11/60
G01N 33/68
G06T 7/00

Abstract:

Disclosed herein are methods of protein quantification and normalization using haloalkylated tryptophan fluorescence. Complex protein samples, i.e., samples that each contain 1,000 or more distinct proteins, from diverse sources that do not have common protein profiles are treated with a halo-substituted organic compound (i.e. haloalkane) that reacts with tryptophan residues to form fluorescent products. Irradiation of the samples with ultraviolet light and the detection and quantification of the resultant fluorescent emissions from all proteins in each sample are then used to obtain comparative values for total protein content among the various samples. The values thus obtained are found to be valid indications of comparative total protein content, despite the fact that the tryptophan levels vary widely among the various proteins in any single sample and the samples, due to the diversity of their origins, tend to differ among themselves in the identities and relative amounts of the proteins that they contain. Protein samples are also normalized to correct for differences in sample dilution, sample loading, and protein transfer inconsistencies, by using stain-free detection of total protein in each of the samples, or detection of subsamples within each sample.

US Patent:

20170030922, Feb 2, 2017

Filed:

Oct 13, 2016

Appl. No.:

15/293154

Inventors:

- Hercules CA, US
Ning Liu - Fremont CA, US
Kevin McDonald - Novato CA, US
Aran Paulus - San Jose CA, US
Anton Posch - Grafing, DE

International Classification:

G01N 33/68
G06T 7/00
G06T 11/60
G06F 19/10

Abstract:

Disclosed herein are methods of protein quantification and normalization using haloalkylated tryptophan fluorescence. Complex protein samples, i.e., samples that each contain 1,000 or more distinct proteins, from diverse sources that do not have common protein profiles are treated with a halo-substituted organic compound (i.e. haloalkane) that reacts with tryptophan residues to form fluorescent products. Irradiation of the samples with ultraviolet light and the detection and quantification of the resultant fluorescent emissions from all proteins in each sample are then used to obtain comparative values for total protein content among the various samples. The values thus obtained are found to be valid indications of comparative total protein content, despite the fact that the tryptophan levels vary widely among the various proteins in any single sample and the samples, due to the diversity of their origins, tend to differ among themselves in the identities and relative amounts of the proteins that they contain. Protein samples are also normalized to correct for differences in sample dilution, sample loading, and protein transfer inconsistencies, by using stain-free detection of total protein in each of the samples, or detection of subsamples within each sample.

US Patent:

20160216255, Jul 28, 2016

Filed:

Jan 21, 2016

Appl. No.:

15/003536

Inventors:

- Hercules CA, US
Ning Liu - Hercules CA, US

International Classification:

G01N 33/543

Abstract:

Immunoblotting systems and method are provided. In one embodiment, the method may be achieved by applying an antibody solution to a surface of a membrane having an optically detectable protein and a target protein transferred thereon, wherein the application of the antibody solution is guided by a signal emitted from the optically detectable protein; and detecting the target protein. Systems and other methods are also described and illustrated.

US Patent:

20150044144, Feb 12, 2015

Filed:

Mar 6, 2013

Appl. No.:

14/376434

Inventors:

- Pleasanton CA, US
Ning Liu - Fremont CA, US

Assignee:

RAYMAT MATERIALS, INC. - Pleasanton CA

International Classification:

A01N 25/00
A61K 33/20
A61K 47/02
A01N 59/00

US Classification:

424 103

Abstract:

The present invention relates to a disinfectant solution, comprising an oxidative and reductive potential (ORP) solution and a colorant. A disinfectant article, a method of forming the disinfectant solution and use of the disinfectant solution are also provided.