Reuben S Harris

US Patent:

20110218158, Sep 8, 2011

Filed:

Sep 22, 2009

Appl. No.:

13/120381

Inventors:

Reuben S. Harris - Saint Paul MN, US
Ming Li - St. Paul MN, US

International Classification:

A61K 31/7135
C12Q 1/70
A61K 31/473
A61K 31/15
A61K 31/192
A61K 31/05
A61K 31/351
A61K 31/165
A61K 31/24
A61K 31/197
A61K 31/135
A61K 31/55
A61K 31/4365
A61K 31/407
A61K 31/352
A61K 31/433
A61K 31/41
A61K 31/137
A61K 31/4164
A61P 31/18

US Classification:

514 23, 435 5, 514284, 514645, 514570, 514733, 514460, 514630, 514538, 514565, 514734, 514657, 51421702, 514301, 514410, 514456, 514361, 514359, 514653, 514392

Abstract:

Cytosine deaminase inhibitors and methods for identifying inhibitors of the anti-retroviral activity of APOBEC3G are described.

US Patent:

20170292931, Oct 12, 2017

Filed:

Mar 30, 2017

Appl. No.:

15/474652

Inventors:

- Minneapolis MN, US
Reuben Harris - Saint Paul MN, US

International Classification:

G01N 27/447
G01N 33/543
B08B 3/04

Abstract:

The present disclosure describes an apparatus including a waste compartment, a membrane compartment on the waste compartment, and a wash buffer reservoir on the membrane compartment. The membrane compartment includes a membrane compartment outlet aperture and is configured to hold at least one membrane, and the membrane compartment outlet aperture fluidly connects the membrane compartment and the waste compartment. The wash buffer reservoir comprises a wash buffer reservoir outlet aperture, and the wash buffer reservoir outlet aperture fluidly connects the wash buffer reservoir and the membrane compartment. The apparatus may be used to wash membranes used in analytic techniques, such as membranes for gel electrophoresis.

US Patent:

20140275224, Sep 18, 2014

Filed:

Nov 3, 2011

Appl. No.:

13/883267

Inventors:

Reuben S. Harris - Saint Paul MN, US
Daniel A. Harki - Minneapolis MN, US
Michael A. Carpenter - Minneapolis MN, US
Ming Li - Saint Paul MN, US

Assignee:

Regents of the University of Minnesota - St. Paul MN

International Classification:

C07D 417/14
A61K 31/4709
C07D 417/12
C07D 235/28
A61K 31/4184
A61K 31/4439
A61K 31/428
C07D 235/18
C07D 277/66
A61K 31/433
C07D 285/08
A61K 31/713
C07D 277/74

US Classification:

514 44 R, 546167, 5483071, 544135, 5483107, 548179, 548129, 5462687, 435184

Abstract:

Compounds and methods are provided for enhancing or boosting the transfection rate or efficiency of mammalian cells by foreign DNA, such as bacterial plasmid DNA. Compounds, including natural products and inventive synthetic compounds can increase the effectiveness of uptake and incorporation of foreign DNA by mammalian cells, such as human cells, by suppression of DNA cytosine deamination, which is believed to be a mechanism by which these cells eliminate foreign DNA. Inhibition of the cytosine deaminase enzymes by compounds as described herein serves to provide more effective transfection of eukaryotic cells by plasmids including engineered gene sequences. Transfection can be used to study cellular processes, or to cure genetic diseases in human patients. The inventive materials and methods increase the efficiency and effectiveness of such transfection techniques.