Stephanie R Mitchell

US Patent:

20120082984, Apr 5, 2012

Filed:

May 28, 2010

Appl. No.:

13/322787

Inventors:

Stephanie L. Mitchell - Somerville MA, US
Jonas M. Winchell - Lilburn GA, US

Assignee:

The Govt. of U.S.A. as represented by the Secretary of the Dept.of Health and Human Services - Atlanta GA

International Classification:

C12Q 1/68
C07H 21/04

US Classification:

435 611, 435 612, 435 615, 536 2433, 536 2432

Abstract:

Methods of detecting , including differentiating between species of and/or strains of are disclosed, for example to detect and genotype a infection. A sample suspected of containing a nucleic acid of a , is screened for the presence of that nucleic acid. The presence of the nucleic acid indicates the presence of the bacterium. Determining whether a nucleic acid is present in a sample can be accomplished by detecting hybridization between a specific primer, a specific primer, and/or a genotype-specific primer and the nucleic acid containing sample. Thus, primers for the detection, species-specific and/or genotype-specific identification of are disclosed. Kits that contain the disclosed primers also are disclosed.