Sujata S Jha

Sujata Jha

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Sujata Jha

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Sujata Jha Cleveland, OH

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Work:

CLEVELAND STATE UNIVERSITY

Jun 2012 to 2000
Graduate Research Assistant CLEVELAND STATE UNIVERSITY
Cleveland, OH
Jan 2008 to May 2014
STUDENT MENTORING CONFERENCE ACIVITY

May 2013 to May 2013
Presenter CLEVELAND STATE UNIVERSITY
Cleveland, OH
Jan 2008 to May 2012
Teaching Assistant

Education:

Cleveland State University
2007 to 2014
Ph.D. in Biology The Maharaja Sayajirao University
2005 to 2007
M.S. in Microbiology The Maharaja Sayajirao University
2002 to 2005
B.S. in Zoology

Skills:

Protein expression and Protein Biochemistry In vitro translation of protein using different translation efficient extracts (Prokaryotic and Eukaryotic ) Site-directed fluorescent labeling of protein using fluorescent tRNAs Expertise in preparation of translation efficient extract from E.coli, HeLa and MEF(Mouse Embryonic Fibroblast) cell lines Experience with handling mammalian cell cultures Protein purification after expression in heterologous host and also from natural source Expertise in using KTA Purifier FPLC for protein purification (Size Exclusion Chromatography, Immobilized Metal Affinity Chromatography and Ion Exchange Chromatography) Protein sample preparation for X-ray Crystallography and 2-D NMR analysis Studying stability and conformation of proteins by employing techniques like limited proteolysis, Differential Scanning Calorimetry (DSC) Sucrose gradient centrifugation and fractionation for isolation of the different ribosomal populations and their associated mRNAs or proteins Electrophoresis: SDS PAGE ( Tris-Tricine and Tris-Glycine) Immuno-methods: ELISA, Immuno precipitation, Western Transfer and Immunoblotting Expertise in the use and handling of radioactive material following all the proper environmental and safety guidelines RNA techniques In vitro transcription, Electrophoretic Mobility Shift Assay (EMSA) Denaturing agarose and acrylamide gels Northern Analysis General Molecular biology techniques Expertise in techniques like purification and quantification of RNA/DNA/protein, PCR, Site-directed mutagenesis, Bacterial culture, Cloning, Transformation, Agarose gel electrophoresis, Luciferase reporter assay, Transient transfection Familiarity with software used in Molecular biology Swiss PDB Viewer, Microcal Origin, GraphPad Prism, ImageQuant, ImageJ, Vector NTI, Unicorn 5.0