Thuc Phuong Le

US Patent:

20020182603, Dec 5, 2002

Filed:

Apr 20, 2001

Appl. No.:

09/839588

Inventors:

William Chapman - San Leandro CA, US
Thuc Le - Milpitas CA, US
Leonard Klevan - Orinda CA, US

International Classification:

C12Q001/68
G01N033/53
G01N033/542
G06K009/00
A61L002/00

US Classification:

435/006000, 435/007900, 427/002110, 382/128000

Abstract:

Methods for fabricating functionalized substrate surfaces for use in preparing biomolecular microarrays, such that the substrate surfaces feature a uniform distribution of attachment functionality, functionalized substrates having such uniform distribution of attachment functionality, and microarrays prepared from such functionalized substrates. A plurality of linker groups are coupled to a substrate surface. A plurality of spacer groups including attachment sites for biological receptors are coupled to the linker groups. The linker groups can be coupled to the surface using a gas phase reaction. Spacers can include polyfunctional linear, branched or dendritic structures, such as polyethylene glycols and Starburst™ dendrimers. Attachment sites can be activated for the attachment of biological receptors.