Skills:
Cell biology: primary cell culture and purification, cell lines, chemical-based and electroporation transfection, cell viability assays, HPLC assay Molecular biology: RT-PCR, PCR, real time PCR, DNA, RNA extraction, protein and nuclear protein extraction (cell and tissue), vector construction, yeast transformation Protein chemistry: SDS-PAGE, Western-blot, protein purification and quantification, Co-IP, enzyme-linked immunosorbant assay (ELISA), Flow cytometry (FACScan) Animal handling: mice, rat and rabbit, IP/IV injection, genotyping Computer Skills: excel in data processing, Microsoft Office, EndNote, SigmaPlot, SigmaStat, Statview, Photoshop Languages: fluent in English, Chinese and Japanese