Xiangjun J Liu

US Patent:

20030082549, May 1, 2003

Filed:

Aug 30, 2001

Appl. No.:

09/943416

Inventors:

Xiangjun Liu - Menomonee Falls WI, US

International Classification:

C12Q001/68
C07H021/04

US Classification:

435/006000, 536/025400

Abstract:

The present invention provides methods and kits for separating and identifying alleles, and thereby the haplotype, in genomic DNA samples. The method generally involves hybridizing primers specific to polymorphic sites within the alleles to the DNA sample, elongating the primers by one or more nucleic acids, separating the elongated primers and identifying the alleles utilizing the elongated primer. The method also allows for a ligation of two primers, their separation and subsequent use in identifying the targeted allele. The method further provides that another primer can be used as a blocking site for elongation of the first primer such that a stretch of DNA that includes a polymorphic site is replicated and identified. The unextended or extended primers can be labeled so that the primer can be easily separated and/or identified.

US Patent:

20040014101, Jan 22, 2004

Filed:

May 5, 2003

Appl. No.:

10/429912

Inventors:

Xiangjun Liu - Menomonee Falls WI, US
Lu Wang - Shorewood WI, US
Daniel Ramon - Brown Deer WI, US

Assignee:

Pel-Freez Clinical Systems, Inc.

International Classification:

C12Q001/68

US Classification:

435/006000

Abstract:

The present invention provides methods for analyzing polymorphic nucleic acids using duplex separation and/or identification techniques. In the present methods one of the nucleic acids has a sequence which includes universal bases that correspond in position to one or more of the polymorphic positions of the polymorphic nucleic acid. The nucleic acid including the universal bases can either represent the polymorphic nucleic acid and hybridize with a reference strand, or act as a reference strand and hybridize with the polymorphic nucleic acid directly, to form a duplex. Separation and/or identification of the duplex from other duplexes and materials can then be performed.

US Patent:

20060008826, Jan 12, 2006

Filed:

Jun 17, 2005

Appl. No.:

11/155424

Inventors:

Xiangjun Liu - Menomonee Falls WI, US

International Classification:

C12Q 1/68

US Classification:

435006000

Abstract:

The present invention provides methods and kits for separating and identifying alleles, and thereby the haplotype, in genomic DNA samples. The method generally involves hybridizing primers specific to polymorphic sites within the alleles to the DNA sample, elongating the primers by one or more nucleic acids, separating the elongated primers and identifying the alleles utilizing the elongated primer. The method also allows for a ligation of two primers, their separation and subsequent use in identifying the targeted allele. The method further provides that another primer can be used as a blocking site for elongation of the first primer such that a stretch of DNA that includes a polymorphic site is replicated and identified. The unextended or extended primers can be labeled so that the primer can be easily separated and/or identified.