Xing Liu

US Patent:

20150096080, Apr 2, 2015

Filed:

Dec 15, 2014

Appl. No.:

14/570823

Inventors:

- Indianapolis IN, US
Vaka S. Reddy - Aurora CO, US
William Michael Ainley - Carmel IN, US
D. Ry Wagner - Pleasant Hill OR, US
Xing Liang Liu - Lake Oswego OR, US

Assignee:

DOW AGROSCIENCES LLC - Indianapolis IN

International Classification:

C12N 15/82
C12Q 1/68

US Classification:

800294, 800278, 800298, 435419, 506 9

Abstract:

Disclosed herein is an activation tagging construct for maize, resulting tagged populations and plants. In one example, an activation tagging DNA construct includes a coding sequence for a transposase, a detectable reporter (such as anthocyanin regulatory genes B-Peru and C1) and a non-autonomous transposable T-DNA cassette. For example, the transposable T-DNA cassette is inserted into the detectable reporter encoding region such that the B-Peru and C1 genes express anthocyanins in a cell containing the maize activation tagging DNA construct only upon excision of the transposable cassette. Methods of generating a tagged population of maize plants include transforming a maize plant cell or tissue with the disclosed constructs.