Xue X Gao

US Patent:

20120190038, Jul 26, 2012

Filed:

Oct 8, 2010

Appl. No.:

13/499356

Inventors:

Yi Tang - San Gabriel CA, US
Xue Gao - Los Angeles CA, US
Xinkai Xie - Los Angeles CA, US

Assignee:

THE REGENTS OF THE UNIVERSITY OF CALIFORNIA - Oakland CA

International Classification:

C12P 17/06
C12N 15/54
C12N 15/63
C12Q 1/68
C12N 1/15
C12N 1/19
C12N 9/96
C12N 9/10
C12N 1/21

US Classification:

435 618, 435193, 536 232, 4353201, 43525233, 4352543, 43525411, 43525422, 4352545, 4352546, 435188, 435125

Abstract:

The invention disclosed herein relates to methods and materials for producing simvastatin and related compounds such as huvastatin. In particular, the disclosure teaches that variants of the LovD acyltransferase polypeptide can be engineered to exhibit properties that facilitate their use in the production of simvastatin and/or huvastatin. The materials and processes disclosed herein are designed so that fermentation facilities currently producing lovastatin can be converted to producing simvastatin and related compounds with minimal modifications.