This invention relates to methods and compositions for the production of glycoconjugates. In particular, organisms are provided with at least one heterologous gene encoding an enzyme for regenerating a sugar nucleotide along with at least one glycosyltransterase. Such organisms are useful for the large-scale synthesis of glycoconjugates.
Methods And Kits For Detection Of 11-Dehydro-Thromboxane B2
- Carlsbad CA, US Kenneth A. Browne - Carlsbad CA, US Ziye Liu - Carlsbad CA, US
International Classification:
G01N 33/558 G01N 33/70 G01N 33/94
Abstract:
Methods, compositions and kits for quantitatively determining specified amounts of 11dhTxB2 in microliter to milliliter quantities of a given sample, wherein, the sample is a biological fluid. Specifically, the biological fluid is a quantity of 1 ml or less of urine from a human subject. The methods may be in the form of consolidated assays that can be run in a high throughput, automation format, such as an enzyme-linked immunosorbent assay (ELISA). Further, the ELISA may be modified into a chemiluminescence assay in order to increase sensitivity and linear range and to reduce the reaction time.