James A. Miller - Agoura CA Philip K. Hsieh - Thousand Oaks CA Larry B. Tsai - Agoura CA
Assignee:
Amgen Inc. - Thousand Oaks CA
International Classification:
C12P 2102 C12P 2106 C07K 740 C07K 710
US Classification:
435 691
Abstract:
A novel method for refolding reduced IGF-I is provided in accordance with the present invention. The method is carried out by providing additional positive charge at the amino terminal end of the IGF-I molecule by the presence of a short leader sequence. The additional positive charge on the met-end of the IGF-I molecule has been found to enable recombinant IGF-I to refold simply by stirring solubilized inclusion body protein for between 2-16 hours, or overnight. The present invention also provides a novel fusion protein intermediate of IGF-I attached to the positively charged leader sequence.
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Philip Hsieh
Education:
University of Southern California - CS(GD), National Central University - CSIE